Bone Marrow and Stem Cell Transplantation

The second edition of Bone Marrow and Stem Cell Transplantation expands upon the previous edition with current, detailed methods on HLA, minor-HLA and Killer Immunoglobulin Like Receptor typing. With new chapters on immunophenotyping and functional characterization of stem cells are included. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Bone Marrow and Stem Cell Transplantation, Second Edition serves as a guide in the application of molecular methods for routine or investigational purposes.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Inhalt

Reporter Gene Technologies for Imaging Cell Fates in Hematopoiesis .- Flow Cytometry for Hematopoietic Cells.- Isolation and Characterization of Mesenchymal Stem Cells.- Methods for Functional Analysis of Stem Cells.- An Overview of HLA Typing for Heamatopoietic Stem Cell Transplantation.- HLA Typing with Sequence-Specific Oligonucleotide Primed PCR (PCR-SSO) and Use of the Luminex™ Technology.- Sequence Based Typing of HLA: An Improved Group Specific Full Length Gene Sequencing Approach.- Molecular Typing Methods for Minor Histocompatibility Antigens.- Natural Killer Cells and Killer-cell Immunoglobulin-like Receptor Polymorphisms: Their Role in Hematopoietic Stem Cell Transplantation.- Methods of Detection of Immune Reconstitution and T Regulatory Cells by Flow Cytometry.- Molecular Methods Used for Detection of Minimal Residual Disease Following Hematopoietic Stem Cell Transplantation in Myeloid Disorders.- Molecular Methods for Detection of Minimal Residual Disease Following Transplantation in Lymphoid and Plasma Cell Disorders.- Molecular Methods for Detection of Invasive Fungal Infections and Mycobacteria and Their Clinical Significance in Hematopoietic Stem Cell Transplantation.- Post-transplant Monitoring of Chimerism by Lineage-specific Analysis.- Urinary Proteomics Employing Capillary Electrophoresis Coupled to Mass Spectrometry in The Monitoring Of Patients After Stem Cell Transplantation.