Carbohydrates Analysis

A new method for the capillary zone electrophoretic separation of neutral carbohydrates with direct UV detection based on the enolization and chelation reactions with phosphates and copper (II) respectively are demonstrated. Using two different electrolytes first consisting of sodium hydroxide and disodium hydrogenphosphate and second consisting of copper(II) sulphate and ammonia, UV absorption at 270 nm results from the enolization and chelation of phosphate and Cu (II) by the carbohydrates under strong alkaline conditions. Neutral monosaccharide and mixture of mono- and di-saccharides were simultaneously separated.Moreover appropriate methods were applied to detect polysaccharides, glucidex 12 and glucidex 17.

Autorentext

Mehmood Babar was born in Jhelum, Pakistan on 12 Oct 1987. After completing his BSc (Hons) from University of Agriculture, Faisalabad, Pakistan, he went on to University of Huddersfield, UK where he completed Masters in Nutrition & Food Sciences with Distinction.