Cerebral Cortex Development

This volume explores the latest developments in the study of the cerebral cortex. The chapters in this book cover a wide array or established methods such as in situ hybridization of brain slices; two-photon FRET/FLIM imaging of cerebral neurons; BioID analysis of actin-binding proteins; generation of iPS cells using Sendai Virus vectors; in vivo whole-cell recording from the mouse brain; and locomotor assays in Drosphila larvae and adult flies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and thorough, Cerebral Cortex Development: Methods and Protocols is a valuable resource that will help both expert and novice researchers further enhance their understanding of this important field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
Overview: Research on the Genetic Architecture of the Developing Cerebral Cortex in Norms and Diseases.- In Situ Hybridization of Brain Slices.- Immunohistochemistry of Brain Tissue.- Two-Photon FRET/FLIM Imaging of Cerebral Neurons.- Pre- and Post-Embedding Immunoelectron Microscopy to Analyze the Central Nervous System.- Mouse Brain Tissue Preparation for Scanning Electron Microscopy.- Simple Method for the Preparation of the Postsynaptic Density Fraction from Mouse Brain.- Reconstitution of Neuronal Motor Traffic on Septin-Associated Microtubules.- BioID Analysis of Actin-Binding Proteins.- Proteomic Analyses of the Mouse Brain.- Generation of iPS Cells using Sendai Virus Vectors.- Directed Differentiation of Neurons from Human iPSCs for Modeling Neurological Disorders.- Generating Homogeneous Brain Organoids from Human iPSCs.- Primary Culture of Dissociated Neurons from Embryonic Cerebral Cortex.- Immunocytochemistry of Primary Cultured Cerebral Cortical Neurons.- Brain Sample Preparation after Performing In Utero Electroporation to Proliferating and Differentiated Cells in the Developing Mouse Neocortex.- Live Imaging of Migrating Neurons and Glial Progenitors Visualized by In Utero Electroporation.- Ca2+ Imaging in Immature Cortical Neurons.- In Vitro Patch-Clamp.- In Vivo Whole-Cell Recording from the Mouse Brain.- In Vivo Brain Microdialysis.- CT-Scan Data Analysis in Malformations of Cortical Development.- MRI Data Analysis in Malformations of Cortical Development.- Genomic Copy Number Analysis using Droplet Digital PCR: A Simple Method with EvaGreen Single-Color Fluorescent Design.- Locomotor Assays in Drosophila Larvae and Adult Flies.- Analyses of Genetic Regulation of the Nervous System in the Nematode Caenorhabditis elegans.- Analyses of Neural Circuits Governing Behavioral Plasticity in the Nematode Caenorhabditis elegans.- Social Behavior in Animal Models of Autism Spectrum Disorder.- Preparation of Healthy Single Neuron or Astrocyte Suspension from Adult Mouse Brain for RNA-seq.