Characterization and Symbiotic Effectiveness of Field pea Rhizobia

This study was initiated to isolate, characterize and evaluate the symbiotic effectiveness of field pea rhizobial isolates from Eastern and Western Hararghae highlands. A total of 25 isolates were induced from soils of each districts. Evaluations of the symbiotic potential of isolates were made in greenhouse on sand culture followed by test of eight effective isolates on soil of Hirna. Experiments were arranged in RCBD with three replications and two controls. Physiological tests were conducted on the eight effective isolates. Presumptive test and cultural characterization result revealed that isolates were root nodule bacteria. 92% of the isolates improved shoot dry weight of the plants over the N- control. Effectiveness rating result also showed that, isolates were effective(1), very effective(23) and ineffective(1). Inoculation results of the eight isolates in soil indicated that the highest dry weight of shoot was recorded by HUFPR20.The isolates showed diverse characteristics in their tolerance of pH, temperature, salt and intrinsic heavy metal resistance. Generally, isolate HUFPR20 is recommended for use as field pea inoculant upon further testing on field condition.

Autorentext

The author was born on May 07, 1985 at Mujja Town in North Wollo Zone of Amhara National Regional State, Ethiopia. He pursued his BEd degree in Biology on July 7, 2006 and Msc in Microbiology on January 21, 2011 at Haramaya University and working in the same university as lecturer and researcher since 2006.