Chemokines: Volume 570

Chemokines, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in chemokines, and includes sections on such topics as chemokine detection using receptors, tracking cellular responses to chemokines, recognition of GAG-bound chemokines, and the production of chemokine receptor complexes for structural and biophysical studies.

Autorentext
Professor, Skaggs School of Pharmacy and Pharmaceutical Sciences and Department of Pharmacology, School of Medicine, University of California, USA

Inhalt

1. Chemokine Detection Using Receptors Immobilized on an Spr Sensor Surface
   José Miguel Rodríguez-Frade, Laura Martínez-Muñoz, Ricardo Villares, Graciela Cascio, Pilar Lucas, Rosa P. Gomariz and Mario Mellado
2. Study of Chemotaxis and Cell-Cell Interactions in Cancer with Microfluidic Devices
   Jiqing Sai, Matthew Rogers, Kathryn Hockemeyer, John P. Wikswo and Ann Richmond
3. Generating Chemokine Analogues with Enhanced Pharmacological Properties using Phage Display
   Karim Dorgham, Fabrice Cerini, Hubert Gaertner, Astrid Melotti, Irène Rossitto- Borlat, Guy Gorochov and Oliver Hartley
4. Methods for the Recognition of GAG-Bound Chemokines
   Pauline Bonvin, Nicolas Fischer and Amanda Proudfoot
5. Monitoring Scavenging Activity of Chemokine Receptors
   Barbara Moepps and Marcus Thelen
6. Dual Color Luciferase Complementation for Chemokine Receptor Signaling
   Kathryn E. Luker and Gary D. Luker
7. Analysis of Arrestin Recruitment to Chemokine Receptors by Bioluminescence Resonance Energy Transfer
   J. Bonneterre, N. Montpas, C. Boularan, C. Galés and N. Heveker
8. Probing Biased Signaling in Chemokine Receptors
   Roxana-Maria Amarandi, Gertrud Malene Hjortø, Mette Marie Rosenkilde and Stefanie Karlshøj
9. Mutagenesis by Phage Display
   Pauline Bonvin, Christine Power, Amanda Proudfoot and Steven Dunn
10. Studying Chemokine Control of Neutrophil Migration in Vivo in a Murine Model of Inflammatory Arthritis
    Yoshishige Miyabe, Nancy D. Kim, Chie Miyabe, and Andrew D. Luster
11. Production of Chemokine/Chemokine Receptor Complexes for Structural and Biophysical Studies
    Martin Gustavsson, Yi Zheng and Tracy M. Handel
12. In Vivo Models to Study Chemokine Biology
    F.A. Amaral, D. Boff and M.M. Teixeira
13. Monitoring Chemokine Receptor Trafficking by Confocal Immunofluorescence Microscopy
    Adriano Marchese
14. Active Shaping of Chemokine Gradients by Atypical Chemokine Receptors: a 4D live Cell Imaging Migration Assay
    Kathrin Werth and Reinhold Förster
15. The Role of Chemokine and Glycosaminoglycan Interaction in Chemokine- Mediated Migration in Vitro and in Vivo
    Irene del Molino del Barrio, John Kirby and Simi Ali
16. Examining Roles of Glycans in Chemokine-Mediated Dendritic-Endothelial Cell Interactions
    Xin Yin, Scott C. Johns, Roland El Ghazal, Catherina L. Salanga, Tracy M. Handel and Mark M. Fuster
17. Preparation and Analysis of N-terminal Chemokine Receptor Sulfopeptides Using Tyrosylprotein Sulfotransferase Enzymes
    Christoph Seibert, Anthony Sanfiz, Thomas P. Sakmar, and Christopher T. Veldkamp
18. Disulfide Trapping for Modeling and Structure Determination of Receptor:Chemokine Complexes
    Irina Kufareva, Martin Gustavsson, Lauren G. Holden, Ling Qin, Yi Zheng and Tracy M. Handel
19. Analysis of G Protein and ?-Arrestin Activation in Chemokine Receptors Signaling
    Alessandro Vacchini, Marta Busnelli, Bice Chini, Massimo Locati and Elena Monica Borroni
20. Flow Cytometry Detection of Chemokine Receptors for the Identification of Murine Monocyte and Neutrophil Subsets
    Ornella Bonavita, Matteo Massara, Achille Anselmo, Paolo Somma, Hilke Brühl, Matthias Mack, Massimo Locati and Raffaella Bonecchi
21. Molecular Pharmacology of Chemokine Receptors
    Raymond H. de Wit, Sabrina M. de Munnik, Rob Leurs, Henry F. Vischer and Martine J. Smit
22. Preparation and Characterization of Glycosaminoglycan Chemokine Co-Receptors
    Nikola Kitic, Martha Gschwandtner, Rupert Derler, Tanja Gerlza and Andreas J. Kungl
23. Production of Recombinant Chemokines and Validation of Refolding
    Christopher T. Veldkamp, Chad A. Koplinski, Davin R. Jensen, Francis C. Peterson, Kaitlin M. Smits, Brittney L. Smith, Scott K. Johnson, Christina Lettieri, Wallace G. Buchholz,Joyce C. Solheim and Brian F. Volkman
24. Quantitative Analysis of Dendritic Cell Haptotaxis
    Jan Schwarz, Michael Sixt