Cilia

This volume is the result of an explosion of molecular-based research on Cilia, which began with the discovery of the universality of intraflagellar transport (IFT) and ciliary genomics/proteomics. The chapters in this book cover topics such as: high resolution imaging and functional characterization of sensory and primary cilia in mammalian cells and zebrafish, methods to study ciliary-mediated chemoresponse in Paramecium , and methods to study centrosomes and cilia in C. elegans and Drosophila . Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and cutting-edge, Cilia: Methods and Protocols is broad and covers motile, sensory, and primary cells. It is a valuable resource to anyone interested in entering the field of ciliary biology using model organisms, including flagellate algae, ciliates, planaria, nematodes, insects, zebrafish, and mammalian cells.

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Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

Methods for Studying Ciliary Import Mechanisms.- Targeting of ASH Domain-Containing Proteins to the Centrosome.- Morphological and Functional Characterization of the Ciliary Pocket by Electron and Fluorescence Microscopy.- Methods to Study Interactions between Ciliogenesis and Autophagy.- Recombinant Reconstitution and Purification of the IFT B Core Complex from Chlamydomonas reinhardtii.- Methods for Studying Movement of Molecules within Cilia.- A FRAP-Based Method for Monitoring Molecular Transport in Ciliary Photoreceptor Cells In Vivo.- Kymographic Analysis of Transport in an Individual Neuronal Sensory Cilium in Caenorhabditis elegans.- Visualization and Manipulation of Cilia and Intraciliary Calcium in the Zebrafish Left-Right Organizer.- Methods for Studying Ciliary-Mediated Chemoresponse in Paramecium.- STED and STORM Superresolution Imaging of Primary Cilia.- CLEM Methods forStudying Primary Cilia.- Methods for Visualization of Neuronal Cilia.- Methods to Study Centrosomes and Cilia in Drosophila.- Analysis of Axonemal Assembly during Ciliary Regeneration in Chlamydomonas.- Planaria as a Model System for the Analysis of Ciliary Assembly and Motility.