Cilia, Part A: Volume 524

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cilia and includes chapters on such topics as electron microscopy of IFT in cilia and flagella, radial spoke isolation and assays, and biomechanical measurements of kinocilium.

Autorentext
Wallace Marshall is an electrical engineer by training, who became interested in biology out of a desire to understand how cells solve engineering problems, such as determining the size of organelles. He received his Ph.D. at UCSF with John Sedat, where he studied the diffusional of motion of interphase chromatin using live cell imaging and computational image analysis. He then trained as a postdoc with Joel Rosenbaum at Yale, where he began studying the mechanisms regulating the length of cilia and flagella. He is now Profess of Biochemistry at UCSF, where he lab continues to study the assembly and length regulation of cilia and flagella, as well as the mechanisms that regulate the size of other organelles. His work takes advantage of an integrated combination of methods including genetics, microscopy, and computational modeling, as well as a wide variety of model organisms including Chlamydomonas reinhardtii, Stentor coeruleus, yeast, flatworms, and mammalian cells.

Klappentext

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cilia and includes chapters on such topics as electron microscopy of IFT in cilia and flagella, radial spoke isolation and assays, and biomechanical measurements of kinocilium

Zusammenfassung
Covering cilia, this title includes chapters on such topics as methods for studying ciliary polarity in Xenopus, analysis of signaling pathways in mammalian spermatozoa, and biochemical and physiological analysis of axonemal dyneins.

Inhalt

1. Electron microscopy of IFT in cilia and flagella

2. Biochemical analysis of ciliary transport
3. Visualization of IFT in Chlamydomonas flagella
4. Visualization of kidney cilia-mediated calcium signaling
5. Methods for analyzing nodal cilia and flow
6. Methods for building synthetic cilia-like motor-microtubule arrays
7. Isolation of IFT particle proteins from Chlamydomonas
8. Radial Spoke isolation and assays
9. Biomechanical measurements of kinocilium
10. Methods for analysis of Calcium/calmodulin signaling in flagella
11. Ubiquitination of Flagellar Proteins
12. Visualizing IFT in C. elegans cilia
13. Proteomic analysis of mammalian primary cilia
14. TSC and TOR signaling in cilia
15. Bioinformatic approaches to cilia
16. Methods for testing cilia function in cell migration
17. Signaling and waveform in Drosophila sperm flagella
18. Analysis of import into primary cilia
19. Patch-Clamp Recording from Mouse and Human Spermatozoa
20. Optical trap measurements of flagellar membrane motility
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