Clinical Applications of Nucleic Acid Amplification

This volume details applications, methods, and protocols on nucleic acid amplification. Chapters detail the management of organ and stem cell transplantation, constitutional genetics and genomics, epidemiology, public health, nucleic acid detection, methods for amplification and sequencing, nuclease-assisted mutant allele enrichment, isothermal, and high-throughput sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Clinical Applications of Nucleic Acid Amplification is a valuable resource for both novice and expert scientists in this developing field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
A Brief Practical Guide to PCR.- Managing a PCR Contamination Event in a Molecular Pathology Laboratory.- Next Generation Sequencing Informatic Architecture Considerations.- Nuclease Enrichment and qPCR Detection of Rare Nucleotide Variants.- MYD88 L265P Mutation Detection by ddPCR: Recommendations for Screening and Minimal Residual Disease Monitoring.- Capture Methylation-Sensitive Restriction Enzyme Sequencing (Capture MRE-Seq) for Methylation Analysis of Highly Degraded DNA.- LT-RPA: An Isothermal DNA Amplification Approach for Improved Microsatellite Genotyping and Microsatellite Instability Detection.- Liquid Biopsy for Advanced Cancer: An Amplicon Based Massively Parallel Sequencing Panel Approach to Precision Oncology.- Mate Pair Sequencing: Next Generation Sequencing for Structural Variant Detection.- Cell Subset-Specific Chimerism Testing by Short Tandem Repeats Analysis for Engraftment Monitoring After Hematopoietic Stem Cell Transplantation.- Immune Cell Lineage-Specific Chimerism Testing by Next-Generation Sequencing for Engraftment Monitoring After Allogeneic Hematopoietic Stem Cell Transplantation.- Rapid Whole Genome Sequencing for Diagnosis of Single Locus Genetic Diseases in Critically Ill Children.- EOSAL-CNV for Easy and Rapid Detection of CNVs by Fragment Analysis.- Rapid Cycle and Extreme Polymerase Chain Reaction.- CRISPR-Cas12-Based Diagnostic Applications in Infectious and Zoonotic Diseases.- SARS-CoV-2 Sequencing for Variant Surveillance.- Tuberculosis Diagnosis Using Isothermal Nucleic Acid Amplification in a Paper-and-Plastic Device.- Loop-Mediated Isothermal Amplification on Paper Microfluidic Chips for Highly Sensitive and Specific Zika Virus Detection Using Smartphone.- Ultrasensitive Reverse Transcriptase Loop-Mediated Isothermal Amplification (US-LAMP) Based Detection of Malaria Infection from Dried Blood Spots.