Clinical Proteomics

This second edition *expands upon the previous edition with current, detailed developments in the field and brings together a multi-disciplinary team of leading researchers to provide their latest protocols for clinical proteomics analysis. Written in the highly successful Methods in Molecular Biology* series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and Practical, Clinical Proteomics: Methods and Protocols, Second Edition presents approaches that will serve as a reliable guide to researchers, including clinicians, chemists, molecular biologists, bioinformaticians and computational, biologists, and investigators working on biomarker development.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Klappentext

This second edition *expands upon the previous edition with current, detailed developments in the field and brings together a multi-disciplinary team of leading researchers to provide their latest protocols for clinical proteomics analysis. Written in the highly successful Methods in Molecular Biology* series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and Practical, Clinical Proteomics: Methods and Protocols, Second Edition presents approaches that will serve as a reliable guide to researchers, including clinicians, chemists, molecular biologists, bioinformaticians and computational, biologists, and investigators working on biomarker development.

Inhalt

Biological Sample Collection For Clinical Proteomics - Existing SOPs.- Targeting The Proteome Of Cellular Fractions: Focus on Secretome.- Preparation Of Urinary Exosomes: Methodological Issues For Clinical Proteomics.- Sample Treatment Methods Involving Combinatorial Peptide Ligand Libraries For Improved Proteomes Analyses.- Glycoprotein Enrichment Method Using A Selective Magnetic Nano-Probe Platform (MNP) Functionalized With Lectins.- The Latest Advancements In Proteomic Two-Dimensional Gel Electrophoresis Analysis Applied To Biological Samples.- 2DE Maps In The Discovery Of Human Autoimmune Kidney Diseases: The Case of Membranous Glomerulonephritis.- MALDI-Imaging Mass Spectrometry on Tissues.- Laser Capture Microdissection Of Fluorescently Labeled Amyloid Plaques From Alzheimer's Disease Brain Tissue For Mass Spectrometric Analysis.- Urine Sample Preparation And Fractionation For Global Proteome Profiling By LC-MS.- Methods In Capillary Electrophoresis Coupled To Mass Spectrometry For The Identification Of Clinical Proteomic/Peptidomic Biomarkers In Biofluids.- Quantification Of Proteins In Urine Samples Using Targeted Mass Spectrometry Methods.- Statistical Issues In The Design And Planning Of Proteomic Profiling Experiments.- Integrating Proteomics Profiling Data Sets A Network Perspective.- The European Medicines Agency Experience With Biomarker Qualification.