Controlled Genetic Manipulations

This book offers basic technical information on controlled genetic manipulation, with examples of creative implementation of the methodology. Covers use of Cre-recombinase, methods for introducing genetic material into the brain, use of optogenetics and more.

The current demand for the development of techniques for controlled genetic manipulations is driven by the anatomical and physiological complexity of the brain and by the need for experimental models that can address this complexity through selective manipulation of defined components of the system: specific neuronal populations or selected synapses. In Controlled Genetic Manipulations, expert researchers present detailed chapters to supply basic technical information about controlled genetic manipulations and to provide examples of creative implementation of this methodology when addressing a unique biological problem. Some chapters of the book describe the most recent developments in the basic methodology, which includes use of Cre-recombinase, methods for delivery of genetic material into the brain and the use of optogenetics, whereas other chapters focus on applying these techniques to addressing particular biological questions like structural and functional mapping of neuronal circuits, analysis of specific synaptic connections, modeling or gene therapy of neurological disorders. As part of the Neuromethods series, chapters include key implementation advice that is crucial for getting optimal results.

Authoritative and practical, Controlled Genetic Manipulations serves as a valuable guide to a variety of techniques, provided by many of the pioneers of the approaches.

Provides both basic technical information and examples of creative implementation Supplies readers with the details needed to bring these techniques to their own labs Features key tips from the experts on how best to avoid common pitfalls Includes supplementary material: sn.pub/extras

Inhalt

Regulation of Cre Recombinase: Use of Ligand-Regulated and Dimerizable Cre for Transgenesis.- Genetic Manipulations Using Cre and Mutant loxP Sites.- Using Recombinant Adeno-Associated Viral Vectors for Gene Expression in the Brain.- Using Lentiviral Vectors as Delivery Vehicles for Gene Therapy.- Targeting Neurological Disease with siRNA.- In Utero Electroporation for Cellular Transgenesis in the Developing Mammalian Forebrain.- Single-Cell Electroporation of siRNA in Primary Neuronal Cultures.- Development and Application of Membrane-Tethered Toxins for Genetic Analyses of Neuronal Circuits.- Genetic Methods for Anatomical Analysis of Neuronal Circuits.- Functional Circuitry Analysis in Rodents Using Neurotoxins/Immunotoxins.- Analysis of Neuronal Circuits with Optogenetics.