Correlation Force Spectroscopy for Single Molecule Measurements

This thesis addresses the development of a new force spectroscopy tool, correlation force spectroscopy (CFS) for the measurement of the properties of very small volumes of material (molecular to µm3) at kHz-MHz frequency range. CFS measures the simultaneous thermal fluctuations of two closely-spaced atomic force microscopy (AFM) cantilevers. CFS then calculates the cross-correlation in the thermal fluctuations that gives the mechanical properties of the matter that spans the gap of the two cantilevers. The book also discusses development of CFS, its advantages over AFM, and its application in single molecule force spectroscopy and micro-rheology.

Nominated as an outstanding theses by Virginia Tech Introduces the dynamic new technique for correlation force spectroscopy (CFS) Details the significance of CFS in polymer physics and chemistry Explains the measurement capability in the area of high frequency rheology and single molecule dynamic measurements Includes supplementary material: sn.pub/extras

Autorentext

Milad Radiom received his PhD in Chemical Engineering at Virginia Tech in 2014, after MEng and BSc in Thermal and Fluids Engineering and Mechanical Engineering respectively from Nanyang Technological University and Amirkabir University of Technology. Thereafter, he was appointed as a postdoctoral research associate in Laboratory of Colloid and Surface Chemistry, University of Geneva. His research interests are physical chemistry of polymers, colloids and surfaces as related to single molecule force spectroscopy, micro-rheology and surface forces.

Inhalt
Introduction.- Correlation Force Spectroscopy.- Dynamics of Single Molecules.- Microrheology with Correlation Force Spectroscopy.- Development of Colloidal Probe Correlation Force Spectroscopy: Case Study.- Correlation Force Spectroscopy for Single Molecule Measurements.- Single Molecule Force Spectroscopy of Dextran.- Single Molecule Force Spectroscopy of Single-Stranded DNA.- Summary.