CRISPR-Cas Enzymes: Volume 616

CRISPR-Cas Enzymes, Volume 616, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Topics covered in this release include CRISPR bioinformatics, A method for one-step assembly of Class 2 CRISPR arrays, Biochemical reconstitution and structural analysis of ribonucleoprotein complexes in Type I-E CRISPR-Cas systems, Mechanistic dissection of the CRISPR interference pathway in Type I-E CRISPR-Cas system, Site-specific fluorescent labeling of individual proteins within CRISPR complexes, Fluorescence-based methods for measuring target interference by CRISPR-Cas systems, Native State Structural Characterization of CRISRP Associated Complexes using Mass Spectrometry, and more.

Autorentext
Scott Bailey is at Johns Hopkins Bloomberg School of Public Health, USA

Inhalt

1. Predicting and visualizing features of CRISPR-Cas systems
   Matthew A. Nethery and Rodolphe Barrangou
   2. Reconstitution and biochemical characterization of ribonucleoprotein complexes in Type I-E CRISPR-Cas systems
   Yibei Xiao and Ailong Ke
   3. Sortase-mediated fluorescent labeling of CRISPR complexes
   Kaylee E. Dillard, Jeffrey M. Schaub, Maxwell W. Brown, Fatema A. Saifuddin, Yibei Xiao, Erik Hernandez, Samuel D. Dahlhauser, Eric V. Anslyn, Ailong Ke and Ilya J. Finkelstein
   4. Fluorescence-based methods for measuring target interference by CRISPR-Cas systems
   Phong T. Phan, Michael Schelling, Chaoyou Xue and Dipali G. Sashital
   5. Probing Cascade complex composition and stability using native mass spectrometry techniques
   Angela Patterson, Monika Tokmina-Lukaszewska and Brian Bothner
   6. High-throughput determination of in vivo DNA sequence preferences for Cas protein binding using Library-ChIP
   Joseph T. Wade
   7. Live-cell single-particle tracking photoactivated localization microscopy of Cascade-mediated DNA surveillance
   Bartosz Turkowyd, Hanna Müller-Esparza, Vanessa Climenti, Niklas Steube, Ulrike Endesfelder and Lennart Randau
   8. In vitro assembly of thermostable Csm complex in CRISPR-Cas type III/A system
   Kwang-Hyun Park, Yan An and Eui-Jeon Woo
   9. Investigation of the cyclic oligoadenylate signaling pathway of type III CRISPR systems
   Christophe Rouillon, Januka S. Athukoralage, Shirley Graham, Sabine Grüschow and Malcolm F. White
   10. A pipeline for characterization of novel Cas9 orthologs
   Tautvydas Karvelis, Joshua K. Young and Virginijus Siksnys
   11. Preparation and electroporation of Cas12a/Cpf1-guide RNA complexes for introducing large gene deletions in mouse embryonic stem cells
   Lucas Kissling, Asun Monfort, Daan C. Swarts, Anton Wutz and Martin Jinek
   12. Directed evolution studies of a thermophilic Type II-C Cas9
   Travis H. Hand, Anuska Das and Hong Li
   13. Kinetic characterization of Cas9 enzymes
   Mu-Sen Liu, Shanzhong Gong, Helen-Hong Yu, David W. Taylor and Kenneth A. Johnson
   14. Single-molecule FRET studies of Cas9 endonuclease
   Viktorija Globyte and Chirlmin Joo
   15. CRISPR-Cas molecular beacons as tool for studies of assembly of CRISPR-Cas effector complexes and their interactions with DNA
   Vladimir Mekler, Konstantin Kuznedelov, Leonid Minakhin, Karthik Murugan, Dipali G. Sashital and Konstantin Severinov
   16. Adapting dCas9-APEX2 for subnuclear proteomic profiling
   Xin D. Gao, Tomás C. Rodríguez and Erik J. Sontheimer
   17. CRISPR-Cas10 assisted editing of virulent staphylococcal phages
   S.M. Nayeemul Bari and Asma Hatoum-Aslan
   18. Reconstitution of CRISPR adaptation in vitro and its detection by PCR
   Robert D. Fagerlund, Timothy J. Ferguson, Howard W.R. Maxwell, Helen K. Opel-Reading, Kurt L. Krause and Peter C. Fineran