Cultivation of BHK-21 cells in adapted serum-free suspension media

Mammalian cells are the most frequently used hosts for the manufacture of biopharmaceutical proteins and the production of viral vaccines. Quality is a key element in establishing an efficient bioconversion process. In this study, we used the BHK- 21C13 (Baby Hamster Kidney) cell line adapted for suspension cultivation. The use of Fetal Bovine Serum (FBS) is traditionally used and is considered a universal supplement, as it allows growth in various mammalian cell lines; however, the use of FBS presents a risk of prion infection, batch-to-batch variability and an increase in the cost of downstream processing. The aim of this study was to compare the cultivation of BHK-21 C13 cells between two media supplemented with SFB and without SFB, through kinetic studies for static and agitated suspension cultivation using a T-flask, spinner flask and bioreactor, respectively.

Autorentext

Leme,Jaci Magister nauk cis ych na Wydziale Nauk Farmaceutycznych Uniwersytetu w São Paulo - USP. Licencjat z nauk biologicznych na Uniwersytecie Nove de Julho - UNINOVE. Technik wsparcia badä naukowych i technologicznych w Instytucie Butantan w São Paulo. Biolog zarejestrowany w CrBio1 nº089618/01.