Developmental, Physiological, and Functional Neurobiology of the Inner Ear

This volume explores the latest techniques in inner ear development, analysis of its sensory cells, and characterization and manipulation of the central auditory and vestibular pathways. The chapters in this book cover topics such as dissection and imaging of the cochlea; behavioral evaluation of animal models of diseases like tinnitus; hair cell function and regeneration; and recent advances in sequencing technology. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Cutting-edge and comprehensive, Developmental, Physiological, and Function Neurobiology of the Inner Ear is a valuable resource for scientists and researchers interested in learning more about this developing field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
Pou4f3DTR Mice Enable Selective and Timed Ablation of Hair Cells in Post-Natal Mice.- Cochlear Explant Cultures: Creation and Application.- Immunohistochemistry and In Situ mRNA Detection using Inner Ear Vibratome Sections.- Genetic Manipulation of the Embryonic Chicken Inner Ear.- Molecular Tools to Study Regeneration of the Avian Cochlea and Utricle.- An Efficient Method to Detect Messenger RNA (mRNA) in the Inner Ear by RNAscope In Situ Hybridization.- A Manual Technique for Isolation and Single-Cell RNA Sequencing Analysis of Cochlear Hair Cells and Supporting Cells.- Ribosomal Pulldown Assays and Their Use to Analyze Gene Expression in Multiple Inner Ear Cell Types.- In Vivo Analysis of Hair Cell Sensory Organs in Zebrafish: From Morphology to Function.- Electrophysiological Recordings of Voltage Dependent and Mechanosensitive Currents in Sensory Hair Cell of the Auditory and Vestibular Organs of the Mouse.- Biophysical Recording from Adult Hair Cell.- Endocochlear Potential Measures, Local Drug Application, and Perilymph Sampling in the Mouse Inner Ear.- In Vivo Whole-Cell Recording in the Gerbil Cochlear Nucleus.- Measurement of Human Cochlear and Auditory Nerve Potentials.- Strategies for Identification of Medial Olivocochlear Neurons for Patch-Clamp Studies of Synaptic Function using Electrical Stimulation and Optogenetics.- Auditory Brainstem Response (ABR) Measurements in Small Mammals.- Behavioral Models Loudness, Hyperacusis, and Sound Avoidance.