Diagnostic Flow Cytometry in Cytology

The book covers the essential practical techniques of flow cytometry in detail. It is divided into two sections: The first section includes the basic practical techniques of flow cytometry in cytology samples. Chapters under this section provide detailed description of the sampling technique, processing, acquisition of the sample, instrumentation and basic principles of flow cytometry. The second section elucidates clinical applications of flow cytometry. Chapters cover the flow cytometry applications in various haematolymphoid neoplasms, tumors of solid organs and body fluid samples. The flow cytometry findings of different tumors are described with the help of multiple colored cytology microphotographs, flow cytometry graphs, boxes, and tables. In addition, it also describes other ancillary techniques in those neoplastic lesions. The book helps practicing pathologists, technical staff and post graduate students to understand flow cytometry findings of the haematolymphoidneoplasms and solid tumor with special emphasis on cytology along with advanced technique. This book will help the students to interpret flow cytometry graphs.

Provides complete knowledge on flow cytometry and sophisticated modern techniques Includes large number of flow cytometry graphs of all the haematolymphoid tumors Helps to interpret flow cytometry graphs Describes important diagnostic flow cytometry features of different lymphoid tumors Includes many case studies for learning

Autorentext
Pranab Dey is Professor in Department of Cytology and Gynecologic Pathology at Post Graduate Institute of Medical Education and Research (PGIMER), Chandigarh. Professor Dey completed M.D. (pathology) from PGIMER, Chandigarh and FRCPath (cytopathology) from Royal College of Pathologist, London. He has been a Consultant of cytology and gynecologic pathology in PGIMER for last 29 years and Professor since last 11 years. Professor Dey has keen interest in flow cytometry and artificial intelligence and conducted many research projects and has pioneered works on DNA flow cytometry, image morphometry, mono-layered cytology and cytomorphologic findings of various lesions on cytology smears. He is a well published author, has published several books in the field of cytology and gynecologic pathology, 300 publications in international journals; 150 original research works and 30 review articles). Professor Dey is a member of various societies.

Inhalt
Part I: Technical aspects.- Introduction and history of flow cytometry.- Basic principles of flow cytometry and instrumentation.- Specimen preparation and data acquisition.- Interpretation of graphs and quality control.- Fluorescent probes and different useful markers for flow cytometry.- Nuclei acid dye for flow cytometry.- Part II: Applications of flow cytometry.- Application of flow cytometry in lymphoid neoplasms.- Application of flow cytometry in body cavity fluids.- Application of flow cytometry in solid tumors.- Cell cycle analysis, DNA flow cytometry and ploidy analysis.- Future applications of flow cytometry.- Practical cases in flow cytometry.