DNA-Based Markers in Plants

Until very recently genetic maps of higher plants were based almost entirely on morphological and biochemical traits. These maps are rapidly being replaced and/or supplemented with DNA-based marker maps based on the use of powerful new molecular techniques.
The new high precision maps can be developed with comparative ease and rapidity. They have a much higher density of markers, which allows revelation of more and more restricted segments of the genome. One of the many revolutionary aspects of this technology is that linkage between molecular markers and traits of interest often can be detected in a single cross. The ability to hybridize probe after probe to the DNA of the same individuals of a segregating population allows one to pursue the analysis until linkage becomes evident. With morphological and biochemical markers used previously, a separate cross was required to test linkage with each new marker. It was seldom that more than three markers could be tested for linkage with the trait of interest in a single cross because of viability problems. With the new techniques described in this volume, a new gene could be placed on the linkage map within a few days instead of the much longer time required with the previous techniques.
In this book, a group of leading researchers provide background information and the latest versions of DNA-based marker maps for a variety of important crops. These maps illustrate the state of the art today. The progress made during the past five years has been truly phenomenal.

Zusammenfassung
Describes techniques which mean that a new gene could be placed on the linkage map within a few days instead of the much longer time required with previous techniques. This text provides background information and current versions of DNA-based marker maps for a variety of crops.

Inhalt

1. Some concepts and new methods for molecular mapping in plants; B. Burr. 2. RFLP technology; G. Kochert. 3. Constructing a plant genetic linkage map with DNA markers; N.D. Young. 4. Mapping quantitative trait loci; S.J. Knapp. 5. Breeding multigenic traits; C.W. Stuber. 6. Nuclear DNA markers in systems and evolution; R. Whitkus, J. Doebley, J.F. Wendel. Section II: RFLP Maps. 7. Molecular marker maps of major crops species; Introduction; R.L. Phillips, I.K. Vasil. 8. Molecular maps of Alfalfa; E.C. Brummer, C.S. Echt, T.J. McCoy, K.K. Kidwell, T.C. Osborn, G.B. Kiss, G. Csanadi, K. Kalman, J. Gyöoryey, L. Ökrész, A.E. Raczkevy, J.H. Bouton, G. Kochert. 9. An integrated RFLP map of Arabidopsis thaliana; H.M. Goodman, S. Hanley, S. Cartinhour, J.M. Cherry, B. Hauge. 10. RFLP maps of Barley; A. Kleinhofs, A. Kilian. 11. DNA-based marker maps of Brassica; C.F. Quiros, Junguo Hu, J.M. Truco. 12. Genetic mapping in Lettuce; R.W. Michelmore, R.V. Kesseli, E.J. Ryder. 13. RFLP maps of Maize; E.H. Coe, J.M. Gardner. 14. RFLP map of Peanut; T. Halward, H.T. Stalker, G. Kochert. 15. Phaseolus vulgaris the common bean; C.E. Vallejos. 16. RFLP map of the Potato; C. Gebhardt, E. Ritter, F. Salamini. 17. Rice molecular map; S.D. Tanksley. 18. Generation of a genetic map for Sorghum bicolor; J.L. Bennetzen, A. Melake-Berhan. 19. RFLP map of Soybean; R.C. Shoemaker. 20. Tomato molecular map; S.D. Tanksley. 21. RFLP maps of bread Wheat; G.E. Hart. 22. Development of RFLP-based linkage maps in diploid and hexaploid oat (Avena sp.); L.S. O'Donoughe, J.P. Rayapati, S.F. Kianian, M.E. Sorrells, S.D. Tanksley, M. Lee, H.W. Rines, R.L. Phillips. Index.