DNA Electrophoresis

This book details methods used to study DNA dynamics both in live cells and in test tubes. It features step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

In DNA Electrophoresis: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study DNA using electrophoresis as the major approach. A powerful tool that allows separating DNA molecules according to their size and shape, this volume includes methods and techniques such as 2-dimentional gel electrophoresis as the major approach. These include methods and techniques such as 2-dimentional gel electrophoresis, DNA electrophoresis under conditions in which DNA molecules are completely or partially denatured during the runs, Pulse Field Gel Electrophoresis, electrophoresis coupled to fluorescence in situ hybridization, as well as protein-DNA interactions studied using electrophoreses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, DNA Electrophoresis: Methods and Protocols aids scientists in continuing to study DNA dynamics both in live cells and in test tubes.

Details methods that are used to study DNA using electrophoresis Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Inhalt

DNA Electrophoresis: Historical and Theoretical Perspectives.- Basic DNA Electrophoresis in Molecular Cloning: a Comprehensive Guide for Beginners.- Analysis of Branched DNA Replication and Recombination Intermediates from Prokaryotic Cells by Two-dimensional (2D) Native-native Agarose Gel Electrophoresis.- Analysis of DNA Structures from Eukaryotic cells by Two-dimensional Native-native DNA Agarose Gel Electrophoresis.- Directionality of Replication Fork Movement Determined by Two-dimensional Native-native DNA Agarose Gel Electrophoresis.- Native/Denaturing Two-Dimensional DNA Electrophoresis And Its Application To The Analysis Of Recombination Intermediates.- Plasmid DNA Topology Assayed by Two-dimensional Agarose Gel Electrophoresis.- A Neutral Glyoxal Gel Electrophoresis Method for the Detection and Semi-Quantitation of DNA Single-Strand Breaks.- Denaturing Gradient Gel Electrophoresis (DGGE).- Polyacrylamide Temperature Gradient Gel Electrophoresis.- Separation of DNA Oligonucleotides using Denaturing Urea-PAGE.- Pulsed-field Gel Electrophoresis of Bacterial Chromosomes.- Resolution of Budding Yeast Chromosomes using Pulse Field Gel Electrophoresis.- Analysis of DNA Damage Via Single Cell Electrophoresis.- Fluorescence in situ Hybridisation on Electrophoresed Cells to Detect Sequence Specific DNA Damage.- Analysis of Protein-DNA Interactions using PAGE: Band-shift Assays.- Assaying Cooperativity of Protein-DNA Interactions using Agarose Gel Electrophoresis.- DNA Bending by Proteins: Utilizing Plasmid pBendAT as a Tool.- Using PCR coupled to PAGE for Detection and Semi-quantitative Evaluation of Telomerase Activity.