DNA Methylation

Over the past few years, DNA methylation technologies and our knowledge of DNA methylation patterns have been advancing at a breathtaking pace. Due to this fact, DNA Methylation: Methods and Protocols, Second Edition completely revises, updates, and expands upon the popular first edition with the most current novel techniques, easier to use and more refined by the tested experience of leading experts. This revision reflects contemporary study of the subject: the analysis of gene-specific DNA methylation patterns has been complemented by genome-wide approaches, and epigenomics takes a central place. Written in the highly successful Methods in Molecular Biology™ series format, the chapters in this volume present brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, DNA Methylation: Methods and Protocols, Second Edition is the perfect resource for scientists who wish to further our understanding of the molecular processes that determine the genomic DNA methylation landscape and for those who aim to identify and implement DNA methylation-based biomarkers for clinical and diagnostic applications.

Provides an easily accessible reference volume for DNA methylation technologies as written by leading experts in the field Focuses on validated and breakthrough innovations for all major questions of development and disease-related DNA methylation research Details methods usable for every laboratory in the world and ensures the rapid implementation of DNA methylation analysis in the laboratory Comprehensive guide to take biomarkers identified through innovative technologies from the research lab to potential diagnostic and clinical applications Covers a broad range of analysis and detection principles for DNA methylation research that can be individually combined to accommodate the researcher's need Serves as the basis for future development of new high-performance and high-throughput assays for DNA methylation detection Includes supplementary material: sn.pub/extras

Inhalt
DNA Methylation: An Introduction to the Biology and the Disease-Associated Changes of a Promising Biomarker.- Global Methylation Levels.- Quantification of Global DNA Methylation by Capillary Electrophoresis and Mass Spectrometry.- Methyl Group Acceptance Assay for the Determination of Global DNA Methylation Levels.- Methods For Whole Genome Analysis Of Dna Methylation Patterns.- Immunodetection Array.- Methylated DNA Immunoprecipitation (MeDIP).- The MIRA Method for DNA Methylation Analysis.- The Help Assay.- Differential Methylation Hybridization: Profiling DNA Methylation with a High-Density CpG Island Microarray.- Analysis of DNA Methylation by Amplification of Intermethylated Sites (AIMS).- Methylation-Sensitive Representational Difference Analysis (MS-RDA).- Restriction Landmark Genomic Scanning: Analysis of CpG Islands in Genomes by 2D Gel Electrophoresis.- GoldenGate® Assay for DNA Methylation Profiling.- 5?-Azacytidine Expression Arrays.- Gene-Specific Methylation Analysis.- DNA Methylation Analysis by Bisulfite Conversion, Cloning, and Sequencing of Individual Clones.- Identification and Quantification of Differentially Methylated Loci by the Pyrosequencing™ Technology.- Mass Spectrometric Analysis of Cytosine Methylation by Base-Specific Cleavage and Primer Extension Methods.- Melting Curve Assays for DNA Methylation Analysis.- Methylation SNaPshot: A Method for the Quantification of Site-Specific DNA Methylation Levels.- Bio-COBRA: Absolute Quantification of DNA Methylation in Electrofluidics Chips.- Restriction Digestion and Real-Time PCR (qAMP).- MethylQuant: A Real-Time PCR-Based Method to Quantify DNA Methylation at Single Specific Cytosines.- Methylation-Specific PCR.- MethyLight.- Quantification of Methylated DNA by HeavyMethyl Duplex PCR.-Special Applications.- Analysis of Methylated Circulating DNA in Cancer Patients' Blood.- Prevention of PCR Cross-Contamination by UNG Treatment of Bisulfite-Treated DNA.- Profiling DNA Methylation from Small Amounts of Genomic DNA Starting Material: Efficient Sodium Bisulfite Conversion and Subsequent Whole-Genome Amplification.