DNA Nanotechnology

This fully updated volume explores methods to create and use nanostructures for characterizing, targeting, and controlling the behavior of cells. Experts from around the globe present their procedures for designing, preparing, characterizing, and using DNA nanostructures, especially towards biological or materials science applications. Additionally, the study of fundamental aspects of nucleic acids, such as their pairing and stacking energy measured with optical tweezers, as well as the targeted delivery of lipid-encapsulated nucleic acid drugs, is also provided to complete the landscape of nanoscale nucleic acids methods. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, DNA Nanotechnology: Methods and Protocols, Third Edition serves as an ideal resource for researchers working on applications of DNA nanostructures in biotechnology and nanomedicine.

Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts

Inhalt

Synthesis and Characterization of DNA Nanostructures and Their Cellular Uptake.- Rapid and High-Yielding Purification of DNA Self-Assembled Structures by Aqueous Two-Phase System.- Gel-Based Analysis of DNA Nanostructure Biostability.- Method for Non-Covalent Functionalization of DNA Nanostructures with DNA Aptamers.- Topogami: Catenating DNA Origami.- DNA Origami Multicolor Quantum Dot Platforms for Sub-Diffraction Spectral Separation Imaging.- Surface-Assisted Assembly of DNA Origami Lattices on Silicon Wafers.- Targeting Bacterial Cells with DNA Nanostructures.- Cell-Membrane-Anchored DNA Nanoplatform for Programming Cellular Interactions.- Method for Imaging and Quantifying Molecular Tensions at Cell-Cell Junctions Using DNA-Based Fluorescent Probes.- Method for Mapping the Membrane Protein Nanoenvironments Using DNA Nanostructures (NanoDeep).- Method for Detecting Mitochondrial ATP by Hybridization Chain Reaction.- Method for Culturing and Imaging Homogeneous 3D Cancer Spheroids as a Model for the Uptake of DNA Nanostructures.- Modification of DNA Nanostructures with Functional Peptides through Copper-Free Click Chemistry.- Measuring Oligovalent Peptide-Virus Interactions Using Electrically Controllable DNA Nanostructures.- Multiplexed Biomarker Detection Using DNA Payloads: Design, Assembly, and Analysis.- Triggerless Bio-Orthogonal Proximity-Induced PNA Ligation Using 2,5-Dioxopentanyl (DOP) Functionality.- A Robust Post-Insertion Method for the Preparation of Targeted Lipid Nanoparticles.- A Method for the Sensitive Detection of Base Pairing and Stacking Energies for Damaged and Non-Canonical Sequences from Single Molecule Unfolding Experiments.