DNA Topoisomerases

Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Zusammenfassung

Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts

Inhalt

Type IA DNA Topoisomerases: A Universal Core and Multiple Activities.- Topoisomerase I and Genome Stability: The Good and The Bad.- DNA Topoisomerases as Targets for Antibacterial Agents.- DNA Supercoiling Measurement in Bacteria.- DNA Catenation Reveals the Dynamics of DNA Topology during Replication.- Mapping E. coli Topoisomerase IV Binding and Activity Sites.- The Use of Psoralen Photobinding to Study Transcription-Induced Supercoiling.- Immunoprecipitation of RNA:DNA Hybrids from Budding Yeast.- Detection of oriC-Independent Replication in Escherichia coli Cells.- Single-Molecule Magnetic Tweezer Analysis of Topoisomerases.- Synthesis of Hemicatenanes for the Study of Type IA Topoisomerases.- An Assay for Detecting RNA Topoisomerase Activity.- Studying TDP1 Function in DNA Repair.- Topoisomerase II Chromatin Immunoprecipitation.- Analyzing Mitotic Chromosome Structural Defects after Topoisomerase II Inhibition or Mutation.- Monitoring the DNA Topoisomerase II Checkpoint inSaccharomyces cerevisiae.- Studying Topoisomerase 1-Mediated Damage at Genomic Ribonucleotides.- A Fluorescence-Based Assay for Identification of Bacterial Topoisomerase I Poisons.- Fluoroquinolone-Gyrase-DNA Cleaved Complexes.- Detection of Topoisomerase Covalent Complexes in Eukaryotic Cells.- Visualization and Quantification of Topoisomerase-DNA Covalent Complexes Using the Trapped in Agarose Immunostaining (TARDIS) Assay.- Study of Plasmid-Mediated Quinolone Resistance in Bacteria.