ELISA

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Details

Enzyme-linked immunosorbent assay, also called ELISA, enzyme immunoassay or EIA, is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample. The ELISA has been used as a diagnostic tool in medicine and plant pathology, as well as a quality control check in various industries. In simple terms, in ELISA an unknown amount of antigen is affixed to a surface, and then a specific antibody is washed over the surface so that it can bind to the antigen. This antibody is linked to an enzyme, and in the final step a substance is added that the enzyme can convert to some detectable signal. Thus in the case of fluorescence ELISA, when light of the appropriate wavelength is shone upon the sample, any antigen/antibody complexes will fluoresce so that the amount of antigen in the sample can be inferred through the magnitude of the fluorescence. Performing an ELISA involves at least one antibody with specificity for a particular antigen.

Weitere Informationen

  • Allgemeine Informationen
    • GTIN 09786130280246
    • Editor Frederic P. Miller, Agnes F. Vandome, John McBrewster
    • Sprache Englisch
    • Größe H220mm x B152mm x T13mm
    • Jahr 2010
    • EAN 9786130280246
    • Format Fachbuch
    • ISBN 978-613-0-28024-6
    • Titel ELISA
    • Untertitel ELISA, Biochemistry, Immunology, Antibody, Antigen, Medical diagnosis, Quality control, Enzyme, Detergent, Enzyme substrate, Signal (electronics), Chromogenic, Fluorogenic, Assay, ELISPOT, Immunoassay
    • Gewicht 198g
    • Herausgeber Alphascript Publishing
    • Anzahl Seiten 120
    • Genre Biologie

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