Environmental Responses in Plants

This second edition describes up-to-date methodological approaches, ranging from physiological assays to imaging and molecular techniques, to study a wide variety of plant responses to environmental cues. Chapters are divided into four sections detailing protocols to investigate key biological processes underlying plant environmental responses in the model organisms Arabidopsis thaliana and Physcomitrella patens * as well as in different crop species. Written in the format of the highly successful Methods in Molecular Biology* series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, Environmental Responses in Plants, Second Edition aims to be a foundation for future studies and to be a source of inspirationfor new investigations in the field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
Analysis of Plant Root Gravitropism.- Hydrotropism: Analysis of the Root Response to a Moisture Gradient.- Evaluating Root Mechanosensing Response in Rice.- Physiological Analysis of Phototropic Responses to Blue and Red Light in Arabidopsis.- Shoot Branching Phenotyping in Arabidopsis and Tomato.- Ribo-Seq Method to Study Genome-Wide Translational Regulation in Plants.- Assessing Flowering Time Under Different Photoperiods.- Analysis of Phytochrome-Dependent Seed Germination in Arabidopsis.- Rhythmic Leaf and Cotyledon Movement Analysis.- Assessing Plant Pigment Regulation in Circadian Experiments.- Chemical perturbation of Chloroplast Ca2+ Dynamics in Arabidopsis thaliana Suspension Cell Cultures and Seedlings.- Screening for Abiotic Stress Response in Rice.- Etiolated Hypocotyls: A New System to Study the Impact of Abiotic Stress on Cell Expansion.- Assessing Postgermination Development in Arabidopsis thaliana under Abiotic Stress.- Assessing Abscisic Acid-Mediated Changes in Stomatal Aperture Through High-Quality Leaf Impressions.- Evaluation of the Anti-Transpirant Activity of ABA-Receptor Agonists in Monocot and Eudicot Plants.- Quantifying Phytohormones In Vivo with FRET Biosensors and the FRETENATOR Analysis Toolset.- Optical Imaging Resources for Crop Phenotyping and Stress Detection.- Analysis of Innate Immune Responses Against Pathogenic Bacteria in Arabidopsis, Tomato and Barley.- Performing Infection Assays of Sporisorium reilianum f. sp. Zeae in Maize.- A Method to Measure the Damage Caused by Cell-Sucking Herbivores.- RNA Isolation from Nematode-induced Feeding Sites in Arabidopsis Roots Using Laser Capture Microdissection.- High-Throughput Profiling of Root-Associated Microbial Communities.- Analysis of Immunity-Related Oxidative Bursts by a Luminol-Based Assay.