Enzymes of Epigenetics Part B: Volume 574

*Enzymes of Epigenetics: Part B, one of two new volumes in the Methods in Enzymology* series, continues the legacy of this premier serial with quality chapters authored by leaders in the field.

This volume covers research methods that are employed in the study of epigenetic regulation, including structural, biochemical, molecular, biological, cellular, computational, and systems approaches.

Topics include chromatin structure and histones, posttranslational histone modification enzymes and complexes, histone modification binders, DNA modifications and nucleic acid regulators, epigenetic technologies, and small molecule epigenetic regulators and biological connections.

Autorentext
Dr. Marmorstein obtained his Ph.D. in Chemistry from the University of Chicago and, following a postdoctoral fellowship at Harvard University and a faculty appointment at The Wistar Institute, he joined the faculty at the Perelman School of Medicine at the University of Pennsylvania. Dr. Marmorstein is currently a Professor in the Department of Biochemistry and Biophysics and an Investigator in the Abramson Family Cancer Research Institute with adjunct appoints in the Department of Chemistry at the University of Pennsylvania and The Wistar Institute. The Marmorstein laboratory uses a broad range of molecular, biochemical and biophysical research tools centered on X-ray crystal structure determination to understand the chemical basis for the epigenetic regulation of gene expression. The laboratory is particularly interested in gene regulatory proteins and their upstream signaling kinases that are aberrantly regulated in cancer and other age-related disorders such as obesity and Alzheimer's disease, and the use of high-throughput small molecule screening and structure-based design strategies towards the development of protein-specific small-molecule probes of protein function and for development into therapeutic agents.

Inhalt

Epigenetic Technologies

1. Identification and Quantification of Histone PTMs using High-Resolution Mass Spectrometry
   Kelly R. Karch, Simone Sidoli and Benjamin A. Garcia
2. Substrate Specificity Profiling of Histone-Modifying Enzymes by Peptide Microarray
   Evan M. Cornett, Bradley M. Dickson, Robert M. Vaughan, Swathi Krishnan, Raymond C. Trievel, Brian D. Strahl, and Scott B. Rothbart
3. ArrayNinja: An Open Source Platform for Unified Planning and Analysis of Microarray Experiments
   Bradley M. Dickson, Evan M. Cornett, Zachary Ramjan and Scott B. Rothbart
4. Chemical Biology Approaches for Characterization of Epigenetic Regulators
   Dalia Barsyte-Lovejoy, Magdalena M Szewczyk, Panagiotis Prinos, Evelyne Lima-Fernandes, Suzanne Ackloo and Cheryl H. Arrowsmith
5. Mapping Lysine Acetyltransferase-Ligand Interactions by Activity-Based Capture
   David C. Montgomery and Jordan L. Meier
6. Investigating Histone Acetylation Stoichiometry and Turnover Rate
   Jing Fan, Josue Baeza and John M. Denu
7. Rapid Semisynthesis of Acetylated and Sumoylated Histone Analogs
   Abhinav Dhall, Caroline E. Weller and Champak Chatterjee

8. An IF-FISH Approach for Co-Visualization of Gene Loci and Nuclear Architecture in Fission Yeast
   Kyoung-Dong Kim, Osamu Iwasaki and Ken-ichi Noma

   Small Molecule Epigenetic Regulators

9. Biology, Chemistry and Pharmacology of Sirtuins
   Antonio Bedalov, Sarwat Chowdhury and Julian A. Simon
10. Synthesis and Assay of SIRT1 Activating Compounds
    Han Dai , James L. Ellis , David A. Sinclair and Basil P. Hubbard

11. Synthesis and Assays of Inhibitors of Methyltransferases
    Xiao-Chuan Cai, Kanishk Kapilashrami and Minkui Luo

    Epigenetics and Biological Connections

12. Exploring the Dynamic Relationship between Cellular Metabolism and Chromatin Structure using SILAC-Mass Spec and ChIP-Sequencing
    Philipp Mews and Shelley L. Berger
13. Current Proteomic Methods to Investigate the Dynamics of Histone Turnover in the Central Nervous System
    Lorna A. Farrelly, Brian D. Dill, Henrik Molina, Marc R. Birtwistle and Ian Maze
14. ChIP-Sequencing to Map the Epigenome of Senescent Cells using Benzonase Endonuclease
    Taranjit Singh Rai and Peter D. Adams
15. Exploiting Chromatin Biology to Understand Immunology
    John L. Johnson and Golnaz Vahed