Enzymes of Epigenetics: Volume 573

*Enzymes of Epigenetics, one of two new volumes in the Methods in Enzymology* series, continues the legacy of this premier serial with quality chapters authored by leaders in the field.

This volume covers research methods that are employed to study epigenetic regulation and includes structural, biochemical, molecular, biological, cellular, computational, and systems approaches.

Topics include chromatin structure and histones, posttranslational histone modification enzymes and complexes, histone modification binders, DNA modifications and nucleic acid regulators, epigenetic technologies and small molecule epigenetic regulators, and biological connections

Autorentext
Dr. Marmorstein obtained his Ph.D. in Chemistry from the University of Chicago and, following a postdoctoral fellowship at Harvard University and a faculty appointment at The Wistar Institute, he joined the faculty at the Perelman School of Medicine at the University of Pennsylvania. Dr. Marmorstein is currently a Professor in the Department of Biochemistry and Biophysics and an Investigator in the Abramson Family Cancer Research Institute with adjunct appoints in the Department of Chemistry at the University of Pennsylvania and The Wistar Institute. The Marmorstein laboratory uses a broad range of molecular, biochemical and biophysical research tools centered on X-ray crystal structure determination to understand the chemical basis for the epigenetic regulation of gene expression. The laboratory is particularly interested in gene regulatory proteins and their upstream signaling kinases that are aberrantly regulated in cancer and other age-related disorders such as obesity and Alzheimer's disease, and the use of high-throughput small molecule screening and structure-based design strategies towards the development of protein-specific small-molecule probes of protein function and for development into therapeutic agents.

Inhalt

Chromatin Structure and Histones

1. In Vitro Chromatin Assembly - Strategies and Quality Control
   Uma Muthurajan, Francesca Mattiroli, Serge Bergeron, Keda Zhou, Yajie Gu, Srinivas Chakravarthy, Pamela Dyer, Thomas Irving and Karolin Luger
2. Preparation, Crystallization and Structure Determination of Chromatin Enzyme/Nucleosome Complexes
   Robert K. McGinty, Ravindra D. Makde and Song Tan
3. Preparation of Recombinant Centromeric Nucleosomes and Formation of Complexes with Non-Histone Centromere Proteins
   Nikolina Sekulic and Ben E. Black
4. Functional Characterization of Histone Chaperones using SNAP-Tag-Based Imaging to Assess De Novo Histone Deposition
   Camille Clément, Isabelle Vassias, Dominique Ray-Gallet and Geneviève Almouzni

5. Analysis of Nucleosome Sliding by ATP-Dependent Chromatin Remodelling Enzymes
   Coral Y. Zhou and Geeta J. Narlikar

   Posttranslational Histone Modification Enzymes and Complexes

6. In Vitro Activity Assays for Myst Histone Acetyltransferases and Adaptation for High-Throughput Inhibitor Screening
   Cheryl E. McCullough and Ronen Marmorstein
7. Preparation and Biochemical Analysis of Classical Histone Deacetylases
   Alejandro Villagra, Eva Sahakian and Edward Seto
8. Recombinant Preparation, Biochemical Analysis, and Structure Determination of Sirtuin Family Histone/Protein Deacylases
   Benjamin Suenkel and Clemens Steegborn
9. Preparation, Biochemical Analysis and Structure Determination of SET Domain Histone Methyltransferases
   Elisa Bergamin and Jean Francois Couture
10. Expression, Purification and Biochemical Analysis of the LSD1/KDM1A Histone Demethylase
    Benoit Laurent and Yang Shi
11. LSD1 Histone Demethylase Assays and Inhibition
    Dawn Hayward and Philip A. Cole
12. Purification, Biochemical Analysis, and Structure Determination of JmjC Lysine Demethylases
    Swathi Krishnan and Raymond C. Trievel

13. Preparation and Analysis of Native Chromatin Modifying Complexes
    Yannick Doyon and Jacques Côté

    Histone Modification Binders (Readers)

14. Preparation, Biochemical Analysis and Structure Determination of the Bromodomain, an Acetyl-lysine Binding Domain
    Chunyan Ren, Lei Zeng and Ming-Ming Zhou

15. Preparation, Biochemical Analysis and Structure Determination of Methyllysine Readers
    Catherine A. Musselman and Tatiana G. Kutateladze

    DNA Modifications and Nucleic Acid Regulators

16. Quantification of Oxidized 5-Methylcytosine Bases and TET Enzyme Activity
    Monica Yun Liu, Jamie E. DeNizio and Rahul M. Kohli
17. Characterization of how DNA Modifications Affect DNA Binding by C2H2 Zinc Finger Proteins
    Anamika Patel, Hideharu Hashimoto, Xing Zhang and Xiaodong Cheng
18. Crystallographic Studies of Telomerase
    Hunter Hoffman and Emmanuel Skordalakes
19. Detection and Analysis of Long Non-Coding RNAs
    Fan Lai, Ezra Blumenthal and Ramin Shiekhattar
20. Identifying Centromeric RNAs Involved in Histone Dynamics in Vivo
    Delphine Quénet, David Sturgill and Yamini Dalal