Epigenetic Biomarkers and Diagnostics

Epigenetic Biomarkers and Diagnostics comprises 31 chapters contributed by leading active researchers in basic and clinical epigenetics. The book begins with the basis of epigenetic mechanisms and descriptions of epigenetic biomarkers that can be used in clinical diagnostics and prognostics. It goes on to discuss classical methods and next generation sequencing-based technologies to discover and analyze epigenetic biomarkers. The book concludes with an account of DNA methylation, post-translational modifications and noncoding RNAs as the most promising biomarkers for cancer (i.e. breast, lung, colon, etc.), metabolic disorders (i.e. diabetes and obesity), autoimmune diseases, infertility, allergy, infectious diseases, and neurological disorders.

The book describes the challenging aspects of research in epigenetics, and current findings regarding new epigenetic elements and modifiers, providing guidance for researchers interested in the most advanced technologies and tested biomarkers to be used in the clinical diagnosis or prognosis of disease.

Autorentext
Dr. Garcia-Gimenez is a Senior Researcher at the Consortium Center for Biomedical Network Research, the Spanish Institute of Health Carlos III (ISCIII), and the INCLIVA Biomedical Research Institute, in the Dept. of Physiology at the Medicine and Dentistry School at the University of Valencia, Spain. Dr. García-Giménez is founder and CEO of EpiDisease S.L. (Spin-Off of CIBER-ISCIII), which develops the technology based on the patent EP15382319.0, currently in national phases (USTPO, EPO, CIPO and SIPO). EpiDisease received NEOTEC funds from CDTI and the Seal of Excellence from the European Commission in the SME Instrument Phase II H2020. Dr. García-Giménez has received 7 scientific and entrepreneurship national and international awards, including the National Award for Young Entrepreneurs in 2013 (awarded by the Spanish Ministry of Health) and the European Young Researcher from the Free Radical Research Society in 2009 and more recently the Eureka Innovation and Entrepreneurship Award in Melbourne (Australia). Dr. Garcia-Gimenez is a researcher at the University of Valencia. He is early in his career, but has been noticed as an up-and-coming authority in epigenetic biomarker research as it relates to disease, stress, and exercise.

Klappentext

Epigenetic Biomarkers and Diagnostics comprises 32 chapters contributed by leading active researchers in clinical epigenetics. The book is divided into three sections, beginning with the basis of epigenetic mechanisms and descriptions of epigenetic biomarkers that can be used in clinical diagnostics and prognostics. . The second section refers to the importance of the sample quality and ethical issues in clinical epigenetics, with the final section presenting the technologies and methods to discover and analyze epigenetic biomarkers. The book describes the challenging aspects of epigenetic research and current findings regarding new epigenetic elements and modifiers, providing guidance for researchers interested in the most advanced technologies and methodologies used in the clinical diagnosis or prognosis of disease.

Inhalt

Preface
José Luis García-Giménez

1. Epigenetic Biomarkers: New findings, perspectives, and future directions in diagnostics
   José Luis García-Giménez, Toshikazu Ushijima, and Trygve O. Tollefsbol

2. The importance of Biobanks in epigenetic studies
   Lorena Peiró-Chova, Olga Bahamonde Ponce, Carolina Abril-Tormo, Jacobo Martínez-Santamaría, José Antonio López-Guerrero and Peter H.J. Riegman

3. Epigenetic Mechanisms as Key Regulators in Disease. Clinical Implications
   Abdelhalim Boukaba, José Luis García-Giménez

4. The evolution of new technologies and methods in clinical epigenetics research
   Tandy L. Dolin Petrov and Nicole C. Riddle

5. Biomarkers and methodologies for monitoring epigenetic drug effects in cancer
   Marianne B. Treppendahl, Lasse Sommer Kristensen and Kirsten Grønbæk

6. Genome-wide techniques for the study of clinical epigenetic biomarkers
   Eneda Toska and F Javier Carmona Sanz

7. Sequenom MassARRAY technology for the analysis of DNA methylation. Clinical applications
   Enrique J. Buso and Marisa Iborra

8. The role of Methylation-specific PCR and associated techniques in clinical diagnostics
   Fang Zhao and Bharati Bapat

9. Pyrosequencing and its application in epigenetic clinical diagnostics
   Ana-Maria Florea

10. Mass spectrometry for the identification of post-translational modifications in histones and its application in clinical epigenetics
    Roberta Noberini, Alessandro Cuomo and Tiziana Bonaldi

11. High-throughput analysis of non-coding RNAs: implications in clinical epigenetics
    Valerio Costa, Maria R Matarazzo, Miriam Gagliardi, Roberta Esposito, Alfredo Ciccodicola

12. Circulating non-coding RNAs as clinical biomarkers
    Francesco Russo, Flavia Scoyni, Alessandro Fatica, Marco Pellegrini, Alfredo Ferro, Alfredo Pulvirenti and Rosalba Giugno

13. DNA methylation biomarkers in lung cancer
    Juan Sandoval and Paula Lopez Serra

14. DNA methylation alterations as biomarkers for Prostate Cancer
    João Ramalho-Carvalho, Rui Henrique, Carmen Jerónimo

15. DNA methylation in breast cancer
    Raimundo Cervera, Alberto Ramos, Ana Lluch and Joan Climent

16. DNA methylation in obesity and associated diseases
    Ana B Crujeiras and Angel Díaz-Lagares

17. DNA Methylation Biomarkers in Asthma and Allergy
    Avery DeVries and Donata Vercelli

18. DNA Methylation in Pregnancy Complications
    Deepali Sundrani, Vinita Khot and Sadhana Joshi

19. Epigenetics in Infectious Diseases
    Genevieve Syn, Jenefer M. Blackwell and Sarra E. Jamieson

20. DNA Methylation in Neurodegenerative Diseases
    Sahar Al-Mahdawi, Sara Anjomani Virmouni and Mark A. Pook

21. The histone code and disease: post-translational modifications as potential prognostic factors for clinical diagnosis
    Nicolas G. Simonet, George Rasti and Alejandro Vaquero

22. Histone Post-Translational Modifications and Chromatin Remodelers in Prostate Cancer
    Filipa Quintela Vieira, Diogo Almeida-Rios, Inês Graça, Rui Henrique and Carmen Jerónimo

23. Histone post-translational modifications in breast cancer and their use in clinical diagnosis and prognosis
    Luca Magnani, Annita Louloupi and Wilbert Zwart

24. Histone variants and post-translational modifications in spermatogenesis and infertility
    Juan Ausio, Yinan Zhang and Toyotaka Ishibashi

25. Circulating histones and nucleosomes as biomarkers in sepsis and septic shock
    José Luis García Giménez, Carlos Romá Mateo, Marta Seco Cervera, José Santiago Ibañez Cabellos and Federico V. Pallardó

26. Chromatin landscape and epigenetic signatures in neurological disorders: emerging perspectives for biological and clinical research
    Pamela Milani and Ernest Fraenkel

27. MicroRNAs deregulation in lung cancer and their use as clinical tools
    Paula Lopez-Serra and Juan Sandoval

28. Circulating Nuclei