Epigenetic Methods in Neuroscience Research

This volume presents state-of-the-art methods for reliable detection of epigenetic changes in the nervous system. Epigenetic Methods in Neuroscience Research guides readers through methods for the analyses of chromatin remodeling, transposable elements, non-coding RNAs, such as miRNAs, and circadian oscillations, including: analysis of DNA methylation in neuronal and glial cells or small tissue samples; sensitive method for quantification of alternative methylated forms of cytosines by liquid chromatography/mass spectrometry; affinity-based detection of modified cytosines by immunohistochemistry or methylated DNA immunoprecipitation; chromatin immunoprecipitation, or ChIP; miRNA high-throughput profiling and the in situ detection of miRNA subtle expression in the brain; analysis of genes with alternative 3'UTRs; and the cite-specific delivery of chromatin-modifying drugs. Written in the popular Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory.

Concise and easy-to-use, Epigenetic Methods in Neuroscience Research provides multidisciplinary epigenetic approach to study genome, and neural, plasticity that will help the reader to successfully address the challenges associated with neurodevelopmental, psychiatric, and neurodegenerative disorders.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Zusammenfassung
Epigenetic Methods in Neuroscience Research guides readers through methods for the analyses of chromatin remodeling, transposable elements, non-coding RNAs, such as miRNAs, and circadian oscillations, including: analysis of DNA methylation in neuronal and glial cells or small tissue samples;

Inhalt
Epigenetics: From Basic Biology To Chromatin Modifying Drugs And New Potential Clinical Applications.- Analysis Of Brain Epigenome: A Guide To Epigenetic Methods.- Analysis Of DNA Methylation By Bisulfite Reaction In Neural Cells As An Example Of Orexin Neurons.- Protocol For The Simultaneous Analysis Of Gene DNA Methylation And Mrna Expression Levels In The Rodent Brain.- Simultaneous Quantification Of Global 5mc And 5hmc Levels In The Nervous System Using A HPLC/MS Method.- Protocol For Methylated DNA Immunoprecipitation (Medip) Analysis.- Cell TypeSpecific DNA Methylation Analysis In Neurons And Glia.- Immunohistochemical Detection Of Oxidized Forms Of 5-Methylcytosine In Embryonic And Adult Brain Tissue.- Analysis Of Histone Modifications In Neural Cells As An Example Of Orexin Neurons.- Site-Specific Delivery Of Epigenetic Modulating Drugs Into The Rat Brain.- Mammalian Genome Plasticity: Expression Analysis Of Transposable Elements.- Tools For Analyzing The Role Of Local Protein Synthesis In Synaptic Plasticity.- In Situ Detection Of Neuron-Specific Micrornas In Frozen Brain Tissue Meister.- Protocol For High-Throughput Mirna Profiling Of The Rat Brain.- Metabolites As Clock Hands: Estimation Of Internal Body Time Using Blood Metabolomics.