Evaluation of performance of serological tests

This book describes Evaluation of performance of serological tests for the detection of Foot and Mouth Disease virus antibodies in Kilifi County, Kenya. Objective: To determine the sensitivity of assays Anigen © FMD NSP Ab ELISA -Korea, NSP Priocheck® kits with Liquid Phase Blocking Enzyme Linked Immunosorbent Assay compared with Virus neutralization test ( the gold standard) and Identification of prevalent serotypes of foot and mouth disease virus in Kilifi County coast of Kenya 2014. Design: cross-sectional study design. Setting of the study: Kilifi County. Subjects: 421 cattle serum. Results: The results showed that both Anigen © FMD NSP Ab ELISA (Korea), NSP Priocheck® Netherlands assays had low sensitivity but high specificity. Liquid Phase Blocking ELI SA had the highest sensitivity 100%. LPBE had a Positive Predictive Value (PPV) =100%, Anigen © FMD NSP Ab ELISA-Korea 94.44% and NSP Priocheck® Netherlands had the lowest 44.74% compared with the VNT 100%. The Cohen's kappa coefficient showed a perfect agreement level of 90%, 93%, 100 and 100% respectively. Conclusion: This study showed no significant difference in sensitivity and both structural and non-structural tests.

Autorentext

Nicholas K. Ruuri hat über 10 Jahre Erfahrung in Laborarbeit und Datenanalyse. Ich habe einen Master-Abschluss in medizinischer Virologie vom INSTITUTE OF TROPICAL MEDICINE AND INFECTIOUS DISEASES der Jomo Kenyatta University, einen BSc. in medizinischer Laborwissenschaft und einen Bsc. in angewandter Biologie (Mikrooptik). Derzeit arbeitet sie am Alupe University College in Kenia als Labortechnikerin.