Evolving Methods for Macromolecular Crystallography

This volume comprises papers presented at the 2005 edition of the Crystallography of Molecular Biology courses that have been held since 1976 at the Ettore Majorana Centre for Scientific Culture in Erice, Italy. This series of courses is renowned for bringing leaders in the field of macromo- cular crystallography together with highly motivated students, in a beautiful and intimate location that encourages people to interact. The warm and informal atmosphere at these Erice conferences, especially these on cryst- lography, has helped to foster long-term scientific interactions and new int- national friendships that have often lasted for the lifetime of the scientists. The course was financed by NATO as an Advanced Study Institute and by the European Commission as a EuroSummerSchool. The papers span the breadth of material presented in the course, which emphasized the practical aspects of modern macromolecular crystallography and its applications. One must start with crystals: Bergfors showed how to improve initial crystals through seeding, while Byrne discussed the difficult problem of crystallizing membrane proteins. The collection of optimal diffraction data requires both careful preparation of cryo-cooled crystals (Garman) and proper processing of the diffraction images (Leslie). To obtain images of electron density, one needs estimates of the phases of the diffracted spots. Sheldrick presented the background to the sing- wavelength anomalous diffraction (SAD) method, which has been gaining popularity, and McCoy discussed the basis of modern maximum likelihood methods for treating information in experimental phasing.

Arises from the renowned Erice Schools on Macromolecular Crystallography Chapters by leading experts in modern methods of macromolecular crystallography Spans the range from detailed practical advice to advanced analytical techniques

Klappentext

This volume draws on the expertise of leaders in the field of macromolecular crystallography to illuminate the dramatic developments that are accelerating progress in structural biology. Their contributions span the range of techniques from crystallization through data collection, structure solution and analysis. The book shows how modern high-throughput methods are contributing to a deeper understanding of medical problems.

Inhalt
Succeeding with seeding: some practical advice.- Expression, purification, and crystallisationof membrane proteins.- Macromolecular cryo-crystallography.- Processing diffraction data with mosflm.- Sad phasing: basic concepts and high-throughput.- Likelihood-based experimental phasing in phaser.- Stochastic molecular replacement.- Likelihood-based molecular replacement in phaser.- Automated structure determination with phenix.- Density Modification In Main.- Ab initio phasing starting from low resolution.- Structural genomics of mycobacterium tuberculosis: a search for function and new drug targets.- Three-dimensional domain swapping and its relevance to conformational diseases.- Structural bioinformatics: from protein structure to function.- Single-particle imaging.