Exo and Endo 1, 4- -D-Glucanases from Hypocrea nigricans

This book is concerned with establishing the cellulolytic activity of H. nigricans; standardizing their Exo- and endo-cellulase and Glucosidase production abilities over different substrate sources through conventional fermentation methods. The standardization processes also involve optimization of various biophysical factors like Temperature, Spore density of the inoculum, Substrate source etc. The standardization process is further statistically optimized and validated via conventional lab methods. The Cellulases are further proteomically validated via LC-MS/MS analysis for the amino acid composition of enzymes and genomically analyzed by PCR for designing and optimization of recombinant organisms producing the cellulases.

Autorentext

Dr. Sunil Kumar Myla is currently working as Key Account Manager in HiMedia Laboratories Pvt., Ltd. He completed his M.Sc., Ph.D., in Microbiology, from Sri Venkateswara University, Tirupati, and Post Graduation Diploma in Food Safety and Quality Management from Indira Gandhi National Open University.