Experimental Protocols in Biotechnology

This manual provides insights into the theory and practical aspects of several biotechnological and biochemical techniques for plants, protozoa, nematodes, insects and fishes, as well as human samples. The book also covers bioinformatics tools. The manual is an inclusive compilation, explaining techniques for microbial cultures, their diagnostics, DNA barcoding, microscopic techniques, blood analysis, parasite diagnostics through copro-antigens, enzyme analysis with enzyme kinetics, gene expression analysis, in-vivo protein visualization in live animals, geno-toxicity assays, quantification of micro RNAs and LncRNAs in tissue sections, the use of droplet PCR, and in-silico analysis. It provides step-by-step descriptions and details of each methodology, together with the final outcomes and inferences, in a simple and lucid manner, easily reproducible even for beginners. The broad range of techniques covered makes this volume extremely useful in understanding the principles of biotechniques, and simple applications for practical courses.

Offers a broad range of experimental models and working standardized protocols Supplemented with illustrations, figures and flow charts Provides the latest evidence-based approaches to the diagnosis and management of haemoprotozoans and helminth infections Details experimental protocols for the efficient quantification of micro RNAs and LncRNAs Describes a plasmid vector-based technique for in-vivo protein visualization in live animals Covers the principles, protocols, precautions and trouble-shooting for various biochemical techniques

Autorentext

Prof. Neelima Gupta, M.Sc. Gold Medalist, M.Phil., Ph.D. in 1981 (Zoology) from the AMU, Aligarh, obtained her D.Sc. degree (Animal Science) from the MJP Rohilkhand University, Bareilly in 2012. Presently working as Vice Chancellor of CSJM University, Kanpur, India since 2018. Recipient of the Ek Janaki Ammal National Award for Animal Taxonomy (Ministry of Environment & Forests), Highest State Award, Saraswati Samman and Centre of Excellence (Government of UP), Vigyan Ratna Award (Council of Science & Technology, UP). Has worked extensively on parasite taxonomy, aquatic toxicology of the Ramganga and Ganga Rivers, and fish health. Nominated by the British Council, DST and INSA in various academic programs.

Dr. Varsha Gupta received her Ph.D (Life Sciences) from Jawaharlal Nehru University, New Delhi, in 2003. She then joined the National Centre for Plant Genome Research, New Delhi, for her post-doctoral training on plant genomics. Presently working at Chhatrapati Shahu Ji Maharaj University, Kanpur since 2006. She has done extensive work on molecular diagnostics using genomic and proteomic approaches, and has published several research articles and one textbook on "Basic and Applied Aspects of Biotechnology".

Inhalt

Chapter 1. Enzyme Linked Immuno sorbent Assay Detection of Bacterial Wilt Causing Ralstonia solanacearum.- Chapter 2. Diagnosis of Tropical Fascioliasis using Specific Antigen Detection in Faeces of Infected Human Being or Animal Species.- Chapter 3. CMV PromoterDriven Expression and Visualization of Tagged Proteins in Live and Fixed Zebrafish Embryonic Epidermis.- Chapter 4. Detection of Blood Parasites and Estimation of Haematological Indices in Fish.- Chapter 5. Techniques to Conduct Morphological and Molecular Investigations of Nematodes.- Chapter 6. Computational Approaches in Drug Designing and its Application.- Chapter 7. Bacteriophage Control for Pseudomonas Aeruginosa Biofilm Formation and Eradication.- Chapter 8.- Detection of Phospho-proteins (phospho-serine and phospho-threonine) from Thylakoid Membranes using Western Blotting.- Chapter 9. Estimation of Plasma membrane and Tonoplast ATPase activity in Plant Tissues.- Chapter 10. Quantification of Conserved MicroRNA in Plants and Validation of New Targets.- Chapter 11. Isolation of Natural Killer Cells from Mouse Bone Marrow and Spleen.- Chapter 12. Gene Expression Analysis from Human Peripheral Blood Mononuclear Cells.- Chapter 13. Use of Droplet PCR in Biomedical Research.- Chapter 14. Determination of Michaelis-Menten Enzyme Kinetics parameters of Alkaline Phosphatase in clinical sample.- Chapter 15. Novel Technique for the Detection of LncRNAs on Tissue Sections.