Extracellular Vesicles

Extracellular Vesicles, Volume 645 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Genetic labeling of extracellular vesicle exosomes for studying biogenesis and uptake in living mammalian cells, Fluorescent Labeling of Extracellular Vesicles, Isolation of extracellular vesicles from lymph, Transgenic rats for tracking body fluid/tissue-derived extracellular vesicles, Isolation of amniotic extracellular vesicles, Urinary extracellular vesicle isolation, Immunocapture-based ELISA to Characterize and Quantify Extracellular Vesicles in Both Cell Culture Supernatants and Body Fluids, and much more.

Autorentext
Sheila Spada is at Weill Cornell Medical College, NY, USA Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology.

Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals:
OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.

Inhalt
1. Genetic labeling of extracellular exosomes for studying biogenesis and uptake in living mammalian cells * Daniel Levy, Mai Anh Do, Annie Brown, Kyle Asano, David Diebold, Hanzhe Chen, Jiayi Zhang, Brendan Lawler and Biao Lu

• 2. Fluorescent labeling of extracellular vesicles
  * Mehdi Dehghani and Thomas R. Gaborski
• 3. Use of antibody arrays to probe exosome and extracellular vesicle mediated functional changes in cells
  * Grazia Ambrosini, Blake Ebert, Richard D. Carvajal, Gary K. Schwartz and Alex J. Rai
• 4. Analysis of individual extracellular vesicles by imaging flow cytometry
  * Tobias Tertel, André Görgens and Bernd Giebel
• 5. Imaging intercellular interaction and extracellular vesicle exchange in a co-culture model of chronic lymphocytic leukemia and stromal cells by lattice light sheet fluorescence microscopy
  * Sara Elgamal, Federico Colombo, Francesca Cottini, John C. Byrd and Emanuele Cocucci
• 6. Methods to purify DNA from extracellular vesicles: Focus on exosomes
  * Sheila Spada
• 7. Quantification of mtDNA in extracellular vesicles
  * Beata Soltes and Balint Nagy
• 8. Deciphering the total RNA content of extracellular vesicles
  * Maria G. Amorim, Emmanuel Dias-Neto and Diana N. Nunes
• 9. Immunocapture-based ELISA to characterize and quantify exosomes in both cell culture supernatants and body fluids
  * Mariantonia Logozzi, Rossella Di Raimo, Davide Mizzoni and Stefano Fais
• 10. Isolation and characterization of human amniotic fluid-derived exosomes * Samantha Sheller-Miller and Ramkumar Menon
• 11. Human urine exosomes: Another important member of the liquid biopsy family * Antonio De Maio
• 12. Three-dimensional cell models for EV production, isolation, and characterization * Liliia Paniushkina, Elena Grueso Navarro, Xu Chen and Irina Nazarenko
• 13. Transgenic rats for tracking body fluid/tissue-derived extracellular vesicles * Aya Yoshimura, Yoshitaka Tamai and Takahiro Ochiya
• 14. Live tracking of extracellular vesicles in larval zebrafish * Benjamin Mary, Shima Ghoroghi, Vincent Hyenne and Jacky G. Goetz
• 15. Assessment of innate immune response activation following the injection of extracellular vesicles isolated from human cell cultures in zebrafish embryos Marco Cafora, Mirjam Hoxha, Laura Cantone, Valentina Bollati, Anna Pistocchi and Luca Ferrari