Fluorescence Fluctuation Spectroscopy (FFS), Part A: Volume 518

Klappentext This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers fluorescence fluctuation spectroscopy and includes chapters on such topics as time-integrated fluorescence cumulant analysis, pulsed interleaved excitation, and raster image correlation spectroscopy and number and brightness analysis. Zusammenfassung Contains chapters on topics such as Time-integrated fluorescence cumulant analysis! Pulsed Interleaved Excitation! and raster image correlation spectroscopy and number and brightness analysis. Inhaltsverzeichnis 40 Years of FCS: How it all Began Brief Introduction to Fluorescence Correlation Spectroscopy Dual-Color Fluorescence Cross-Correlation Spectroscopy (FCCS) with continuous Laser Excitation in a Confocal Setup Brightness Analysis Time-Integrated Fluorescence Cumulant Analysis and its Application in Living Cells Raster Image Correlation Spectroscopy and Number and Brightness Analysis Global Analysis in Fluorescence Correlation Spectroscopy and Fluorescence Lifetime Microscopy Dual-focus Fluorescence Correlation Spectroscopy (2fFCS) Pulsed Interleaved Excitation: Principles and Applications Image Correlation Spectroscopy: Mapping Correlations in Space, Time and Reciprocal Space

Zusammenfassung
Contains chapters on topics such as Time-integrated fluorescence cumulant analysis, Pulsed Interleaved Excitation, and raster image correlation spectroscopy and number and brightness analysis.

Inhalt

1. 40 Years of FCS: How it all Began

2. Brief Introduction to Fluorescence Correlation Spectroscopy ****

3. Dual-Color Fluorescence Cross-Correlation Spectroscopy (FCCS) with continuous Laser Excitation in a Confocal Setup ****

4. Brightness Analysis ****

5. Time-Integrated Fluorescence Cumulant Analysis and its Application in Living Cells ****

6. Raster Image Correlation Spectroscopy and Number and Brightness Analysis ***

7. *Global Analysis in Fluorescence Correlation Spectroscopy and Fluorescence Lifetime Microscopy ****

8. Dual-focus Fluorescence Correlation Spectroscopy (2fFCS) ****

9. Pulsed Interleaved Excitation: Principles and Applications ****

10. Image Correlation Spectroscopy: Mapping Correlations in Space, Time and Reciprocal Space **