Freezing Processes in Cell Suspensions Evaluated Using Cryomicroscopy

This work was the masters thesis of the author
towards his degree in mechanical engineering from
Louisiana State University, USA.In this work freezing
responses of two different cancer cell types, Jurkat
and HeLa were investigated using the well known
method of cryomicroscopy. The freezing experiments
and subsequent numerical simulations have yielded the
membrane permeability characteristics of these cells.
Certain freezing rates have enabled intracellular ice
formation. The author believes the findings may help
future researchers in developing cryopreservation and
cryosurgical protocols. Freezing experiments were
also performed on Pacific Oyster Embryos(Crassostrea
gigas) and intracellular ice formation was observed.
Overall, this work should be useful to students and
researchers working in the field of bio-heat and mass
transfer.Those interested in learning more about
cryomicroscopy as a method of evaluation of freezing
responses of biological cells and the two significant
freezing phenomena will also find it absorbing. The
author firmly believes that further research in this
area will revolutionize the therapy of cervical and
blood cancer.

Autorentext
Author was born in March 1981 in India. He did his bachelors in production engineering (ranked 4th in university)in 2004.He worked as a research engineer in one of India's largest engine manufacturing companies.He has now completed MS in Mechanical Engineering(CGPA 3.875),and is joining as R&D engineer in an engg & construction co in Houston,USA.

Klappentext
This work was the masters thesis of the author towards his degree in mechanical engineering from Louisiana State University, USA.In this work freezing responses of two different cancer cell types, Jurkat and HeLa were investigated using the well known method of cryomicroscopy. The freezing experiments and subsequent numerical simulations have yielded the membrane permeability characteristics of these cells. Certain freezing rates have enabled intracellular ice formation. The author believes the findings may help future researchers in developing cryopreservation and cryosurgical protocols. Freezing experiments were also performed on Pacific Oyster Embryos(Crassostrea gigas) and intracellular ice formation was observed. Overall, this work should be useful to students and researchers working in the field of bio-heat and mass transfer.Those interested in learning more about cryomicroscopy as a method of evaluation of freezing responses of biological cells and the two significant freezing phenomena will also find it absorbing. The author firmly believes that further research in this area will revolutionize the therapy of cervical and blood cancer.