Functional Glycomics

This reference provides the latest developments and emerging techniques used to study functional glycomics. Chapters included needed materials, reagants, step-by-step protocols, notes sections, common pitfalls to avoid and tips on troubleshooting.

The study of functional glycomics requires the continuous development of rapid and sensitive methods for the identification of glycan structures and integration to structure-function relationships. In Functional Glycomics: Methods and Protocols, a panel of world-renowned experts provide new developments and emerging glycomics techniques in the form of detailed protocols exploring the fundamental challenges and most cutting-edge novel applications. Written in the highly successful Methods in Molecular Biology™ series format, chapters in this volume present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting key tips on troubleshooting and avoiding known pitfalls.

Authoritative and easy to use, Functional Glycomics: Methods and Protocols serves as an ideal reference for scientists working in biochemistry, molecular biology, cell biology, immunology, microbiology, and virology and a guide to appropriate techniques and the design of achievable research plans in this vital field.

Includes the latest developments and emerging state-of-the-art techniques to determine the glycome function Examines problem-driven strategy and methodology for Glycobiology researchers, both advanced and beginners, in academia and industry Provides integrated analysis approach of whole sugar chains Presents a practical manual to identify and monitor modifications and changes in glycomes for biomarker discovery and vaccines Includes supplementary material: sn.pub/extras

Inhalt
Functional Glycomics and Glycobiology: An Overview.- Historical Overview of Glycoanalysis.- Quantitative Glycomics.- Human Gangliosides and Bacterial Lipo-oligosaccharides in the Development of Autoimmune Neuropathies.- Biotinylated Multivalent Glycoconjugates for Surface Coating.- Profiling LPS Glycoforms of Non-typeable Haemophilus influenzae by Multiple-Stage Tandem Mass Spectrometry.- In Vitro Reconstitution of Escherichia coli O86 O Antigen Repeating Unit.- Glycoprotein Characterization.- N-Linked Glycoprotein Analysis Using Dual-Extraction Ultrahigh-Performance Liquid Chromatography and Electrospray Tandem Mass Spectrometry.- Microarray-Based Study of CarbohydrateProtein Binding.- The Application of NMR Spectroscopy to Functional Glycomics.- Metabolomics in Glycomics.- Characterization of Lipid-Linked Oligosaccharides by Mass Spectrometry.- A Glycomics Approach to the Discovery of Potential Cancer Biomarkers.- Extraction of Chondroitin/Dermatan Sulfate Glycosaminoglycans from Connective Tissue for Mass Spectrometric Analysis.- N-Linked Protein Glycosylation in a Bacterial System.- Characterization of Polysaccharides Using Mass Spectrometry for Bacterial Serotyping.- Is Permethylation Strategy Always Applicable to Protein N-Glycosylation Study?: A Case Study on the O-Acetylation of Sialic Acid in Fish Serum Glycans.- Bioinformatics in Glycomics: Glycan Characterization with Mass Spectrometric Data Using SimGlycan™.