Functional Glycomics

Klappentext In the past decade, a tremendous progress in understanding the function of carbohydrates or glycans took place. This progress also starts showing the promise of drug development based on the function of carbohydrates. This critical progress largely owes a development of new methodology to determine a role of a carbohydrate and then develop a method to interfere such biological process, mostly taking a form of protein-carbohydrate interaction. In the current three volumes of Methods in Enzymology focusing on glycomics, readers will appreciate how such discoveries were made and how such methods can be applied for readers own research efforts. Each chapter has been designed so that enough scientific background will be given in each chapter for further development of methods by readers themselves. The books thus should be useful for all levels of scientists starting from the last years of colleges, graduate students, postdoctoral fellows to professors and to all levels of scientists in research institutes including industry. The editor is highly hopeful and confident that these volumes of Methods in Enzymology will contribute to another surge in determining the structure and function of glycans and developing new drugs based on carbohydrate functions. Zusammenfassung A collection focusing on glycomics. It lets readers appreciate how such discoveries were made and how such methods can be applied for readers' own research efforts. It is suitable for different levels of scientists starting from the last years of colleges! graduate students! postdoctoral fellows to professors. Inhaltsverzeichnis Section I. Stem Cell 1. ?4-galactosyltransferase V in growth regulation Jianxin Gu 2. Roles of Polysialic acid in neural stem cells Kiyohiko Angata 3. Chondroitin sulfate in neural stem cells Andreas Faissner 4. Transcription in Stem Cell Kelly Moremen 5. Directing stem cell trafficking through GPS Robert. Sackstein Section II. Gene knockout and mutant mice 6. Cosmc-chaperone Richard Cummings Ju 7. Core 3 O-glycan Lijuan Xia 8. Core3 glycan as tumor suppressor Lee Fukuda 9. Core 4 O-glycan Jamey Marth, Minoru Fukuda 10. b3GlcNAcT2, a major polylacosamine synthase Akira. Togayachi and Hisashi Narimatsu 11. GalNAcT-1 Mari Tenno. Jamey Marth 12. GlcNAcT-IVa-Diabetes Kazuaki Ohtsubo and Jamey Marth 13. Ashwell receptor Prabhijt. Grewal and Jamey Marth 14. Roles ofGlcNAc-6-O-sulfotransferases in lymphoid and non-lymphoid tissues Hiroto Kawashima 15. ?lcNAcT3-core 2GlCNAcT1-selectin ligand Junya. Mitoma and Minoru Fukuda 16. ManII-ManIIX Michiko Fukuda 17. Histochemical analysis carbohydrate antigens in inflammation Motohiro Kobayashi and Jun Nakayama Section III. Alpha-Dystroglycan and tumor 18. Genetic defect in muscular dystrophy Paul Martin 19. POMT1 knockout Sabine Strahl and Jesus Cruces 20. POMGnT1 Mutation in human Tamao and Endo 21. POMGnT1 Knockout mice Huaiyu Hu 22. Large mutation and Large 2. Jane. Hewitt 23. a-dystroglycan glycans as tumor suppressor Xingfeng Bao and Minoru Fukuda 24. Tumor formation assay Shingo Hatakeyama and Chikara Ohyama ...

Zusammenfassung
A collection focusing on glycomics. It lets readers appreciate how such discoveries were made and how such methods can be applied for readers' own research efforts. It is suitable for different levels of scientists starting from the last years of colleges, graduate students, postdoctoral fellows to professors.

Inhalt

Section I. Stem Cell

1. 4-galactosyltransferase V in growth regulation

   Jianxin Gu

2. Roles of Polysialic acid in neural stem cells

   Kiyohiko Angata

3. Chondroitin sulfate in neural stem cells

   Andreas Faissner

4. Transcription in Stem Cell

   Kelly Moremen

5. Directing stem cell trafficking through GPS

   Robert. Sackstein

   Section II. Gene knockout and mutant mice

6. Cosmc-chaperone

   Richard Cummings Ju

7. Core 3 O-glycan

   Lijuan Xia

8. Core3 glycan as tumor suppressor

   Lee Fukuda

9. Core 4 O-glycan

   Jamey Marth, Minoru Fukuda

10. b3GlcNAcT2, a major polylacosamine synthase

    Akira. Togayachi and Hisashi Narimatsu

11. GalNAcT-1

    Mari Tenno. Jamey Marth

12. GlcNAcT-IVa-Diabetes

    Kazuaki Ohtsubo and Jamey Marth

13. Ashwell receptor

    Prabhijt. Grewal and Jamey Marth

14. Roles ofGlcNAc-6-O-sulfotransferases in lymphoid and non-lymphoid tissues Hiroto Kawashima

15. lcNAcT3-core 2GlCNAcT1-selectin ligand

    Junya. Mitoma and Minoru Fukuda

16. ManII-ManIIX

    Michiko Fukuda

17. Histochemical analysis carbohydrate antigens in inflammation

    Motohiro Kobayashi and Jun Nakayama

    Section III. Alpha-Dystroglycan and tumor

18. Genetic defect in muscular dystrophy

    Paul Martin

19. POMT1 knockout

    Sabine Strahl and Jesus Cruces

20. POMGnT1 Mutation in human

    Tamao and Endo

21. POMGnT1 Knockout mice

    Huaiyu Hu

22. Large mutation and Large 2.

    Jane. Hewitt

23. a-dystroglycan glycans as tumor suppressor

    Xingfeng Bao and Minoru Fukuda

24. Tumor formation assay

    Shingo Hatakeyama and Chikara Ohyama