Fungi and Cholesterol Oxidase Enzyme

In this book, the possibility of production of
cholesterol oxidase enzyme from fungal isolates were
studied. These fungi were isolated from raw butter
and food oils. The study focused on four main aims:
a)surveyed the mycobiota in 30 samples of each of
raw butter and food oils by using five types of
isolation media, b) 432 isolates belonging to 31
species and 14 genera were screened for their
abilities to produce cholesterol oxidase enzyme
using two assay techniques, cholesterol clear zone
(CCZ) and spectrophotometrically, C)studying the
effects of some environmental and nutritional
factors on cholesterol oxidase production by
Aspergillus niger MF 75, Geotrichum candidum RM 22
and Penicillium corylophilum AM 99 (from survey
results, these three fungi were the best for enzyme
production), and d)purification and characterization
of cholesterol oxidase enzyme produced by these
isolates.

Autorentext

Mohamed Fadl Allah Abdel-Latef Awad is a researcher interested in Microbiology. One of his most interested fields is the microbial wastewater treatment. Prof. S.I.I. Abdel-Hafez is a professor of Microbiology and he has many publications in Microbiological sciences. Prof. A.A.Barakat & Prof. A.A.El-Shanwany are professors of Micology.

Klappentext

In this book, the possibility of production of cholesterol oxidase enzyme from fungal isolates were studied. These fungi were isolated from raw butter and food oils. The study focused on four main aims: a)surveyed the mycobiota in 30 samples of each of raw butter and food oils by using five types of isolation media, b) 432 isolates belonging to 31 species and 14 genera were screened for their abilities to produce cholesterol oxidase enzyme using two assay techniques, cholesterol clear zone (CCZ) and spectrophotometrically, C)studying the effects of some environmental and nutritional factors on cholesterol oxidase production by Aspergillus niger MF 75, Geotrichum candidum RM 22 and Penicillium corylophilum AM 99 (from survey results, these three fungi were the best for enzyme production), and d)purification and characterization of cholesterol oxidase enzyme produced by these isolates.