G Protein Methods and Protocols

03490-9, Hardcover, April 22, 1997,

G PROTEIN METHODS AND PROTOCOLS: Role of Proteins in Psychiatric and Neurological Disorders
Edited by
Ram K. Mishra, McMaster University, Hamilton, Ontario, Canada
Glen B. Baker, University of Alberta, Edmonton, Canada
Alan A. Boulton, University of Saskatoon, Canada

Combining the cutting-edge insights of leading basic and clinical investigators, G Protein Methods and Protocols provides both critical reviews and detailed protocols for studying the medical role played by G proteins and signal transduction in mental and neurological disease. The psychiatric and neurological disorders covered range from Parkinson's disease and schizophrenia to manic depression, drug addiction, and mood and anxiety disorders. The protocols contain the latest methods and techniques for G protein analysis and application in research related to the central nervous system.

This new work marks a novel departure from books focused narrowly on the physical properties of G proteins in isolation. By providing detailed methods and techniques, this volume's front-line contributors bring out the implications of G protein signal transduction for psychiatric and neurological disorders. Practical and authoritative, G Protein Methods and Protocols will surely prove a major practical resource for all neurochemists, neurologists, molecular biologists, pharmacologists, and psychiatrists seeking to study and better understand these disease states.

Klappentext

The G proteins are a family of structurally homologous, plasma membrane-associated guanine-nucleotide-binding proteins. These proteins play an integral role in the tra- duction of extracellular signals through second messenger systems. As such, G proteins affect a wide variety of intra cellular biochemical reactions by regulating the concent- tion of second messengers in cells. G proteins are heterotrimeric, consisting of a, p, and y polypeptide chains, with G protein specificity largely det- mined by the a-subunit, Molecular cloning of G protein s- units has revealed 23 distinct a-subunits, encoded by 17 different genes. Based on functional measures, G proteins are generally classified into three major categories: the G, family, which is stimulatory for adenylyl cyclase; the G, f- ily, which is inhibitory for adenylyl cyclase; and the G, f- ily, which stimulates phospholipases (Birnbaumer and Birnbaumer, 1995). Alternatively, on the basis of sequence homology, G proteins can be subdivided into four cate- ries: G,, G,, G,, and G12.

Inhalt
In Vitro Autoradiographic Localization of Receptor-Stimulated [35S]GTP?S Binding in Brain.- Small-Molecular-Weight G Proteins.- G Proteins in the Etiology and Treatment of Mental Disorders.- G Protein ?- and ??-Subunits as Possible Mediators of Dopamine-D1:D2 Receptor Ligand Binding Interactions.- Modulation of G Proteins in Rat Striatum by Neuroleptic Drugs and a Peptidomimetic Analog of Pro-Leu-Gly-NH2.- G Proteins and Animal Models of Parkinson's Disease.- Protein Kinase C as a Modulator of 5- HT1A and Dopamine- D2 Receptor Signaling.- Molecular Mechanisms of Opiate Addiction in the Nucleus Accumbens.- G Protein-Linked Opioid Receptors.- G Protein-Coupled melatonin receptors.- G Proteins in the Pathophysiology and Treatment of Mood and Anxiety Disorders.- Determination of Adenosine Receptor-G Protein Coupling.- G Proteins and Mood Disorders.- G Proteins in the Medial Temporal Lobe in Schizophrenia.- G Protein Abnormalities in Schizophrenia.