G Protein Pathways, Part B: G Proteins and Their Regulators: Volume 344

This volume covers topics such as the structure and identification of functional domains of G proteins, and activation of G proteins by receptors or other regulators. The text takes an integrated approach to studying common experimental questions at many different levels related to G proteins. Methods related to G proteins using molecular modeling, systems biology, protein engineering, protein biochemistry, cell biology, and physiology are all accessible in the same volume. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant todaytruly an essential publication for researchers in all fields of life sciences.

Inhalt

Section I: Activation of G Proteins by Receptors or Other Regulators

of G Protein Activation in Sf9 and Mammalian Cells by Agonist-Promoted [35S]GTP?S Binding

Kinetic and Thermodynamic Constants for Interaction of G Protein Subunits and Receptors by Surface Plasmon Resonance Spectroscopy

Localization of Receptor-Activated G Proteins in Brain

and Use of C-Terminal Minigene Vectors for Studying Role of Heterotrimeric G Proteins

Receptor-G Protein Specificity Using Ga Chimeras

of Dominant Negative Mutations in Analysis of G Protein Function in Saccharomyces cerevisiae

Assays for Mammalian G-Protein-Coupled Receptors in Yeast

of G Protein ß? Complex in Receptor-G Protein Interaction

Down-Regulation of G-Protein Coupling: Reconstitution of Phosducin Transducin of cGMP Cascade in Bovine Rod Photoreceptor Cells

of Signal Transfer from Receptor to Go/Gi in Different Membrane Environments and Receptor-Independent Activators of Brain G Protein

of Modulators of Mammalian G-Protein Signaling by Functional Screens in the Yeast Saccharomyces cerevisiae

Section II: Isolation or Production of Native or Modified

of a Subunit of Gs in Escherichia coli

of G Protein Isoforms GOA GOC from Bovine Brain

of Proteins with Methionine Aminopeptidase/or N-Myristoyltransferase in Escherichia coli to Increase Acylation Homogeneity of Protein Preparations

of G Protein ß? from Bovine Brain

and Analysis of G Protein ? Subunits

of G? Prenylcysteine Carboxyl Methyltransferase

and Application of G Protein ? Subunit-Derived Peptides Incorporating a Photoactive Isoprenoid

Section III: Functional Analysis of G Protein Subunits

and Functional Analysis of G Protein a Subunits in S49 Lymphoma Cells

Gene Knockout Knockin Strategies in Application to a Subunits of Gi/Go Family of G Proteins

Cellular Role of Ga12

Regulatable Expression of Activated Heterotrimeric G Protein a Subunits in Transgenic Mice

Tissue-Specific Suppression of Heterotrimeric G Protein a Subunits in Vivo

of Replication Defective Adenovirus That Expresses Mutant Gas Q227L

of Adenovirus-Directed Expression of Activated Gas in Rat Hippocampal Slices

Kinetic Measurement of Individual Reactions of G-Protein-Catalyzed GTPase Cycle

of Genomic Imprinting of Gsa Gene

Localization of G Protein Subunits

Approaches to Study G Protein Mechanisms

G Protein ß? Specificity for Effector Recognition

Suppression of G Protein ? Subunits

Section IV: G Protein Structure and Identification

of Scanning Mutagenesis to Delineate Structure-Function Relationships in G Protein a Subunits

of Gs-Selective Inhibitory Compounds

of Deamidated G Protein Subunits

G Protein Heterotrimer Formation

of Peptide Probes to Determine Function of Interaction Sites in G Protein Interactions with Effectors

Interaction Assays with G Proteins

Traces of Functional Surfaces along G Protein Signaling Pathway

of Ligands for ß? Subunits from Phage-Displayed Peptide Libraries

Protein-Protein Interactions by Peptide Docking Protocols

Characterization of Intact G Protein ? Subunits by Mass Spectrometry

Section V: RGS Proteins and Signal Termination

Assays for GTPase-Activating Proteins

of RGS Proteins in Saccharomyces cerevisiae

of RGS Protein, Sst2, from Saccharomyces cerevisiae and Escherichia coli

Domain: Production and Uses of Recombinant Protein

for Interacting Partners for Gai3 and RGS-GAIP Using the Two-Hybrid System

of RGS Protein Activity in Vitro Using Purified Components

RGS Protein Interactions with Gqa

of Complex Formation between RGS Protein G? Subunit-like Domains and Gß Subunits

Function in Visual Signal Transduction

Cloning of Regulators of G-Protein Signaling Family Members and Characterization of Binding Specificity of RGS 12 PDZ Domain

Author index

Subject Index