Gene Therapy of Solid Cancers

This volume provides insight into recent developments on experimental and clinical strategies for cancer gene therapy. Gene Therapy of Solid Cancers: Methods and Protocols guides readers through *protocols on gene therapeutic strategies in combination with helpful technical notes. Written in the highly successful Methods in Molecular Biology* series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Concise and easy-to-use, Gene Therapy of Solid Cancers: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
Aptamer Targeting the ERBB2 Receptor Tyrosine Kinase for Applications in Tumor Therapy.- Gene Gun Her2/neu DNA Vaccination: Evaluation Of Vaccine Efficacy in a Syngeneic Her2/neu Mouse Tumor Model.- MIDGE Technology for the Production of a Fourfold Gene-Modified, Allogenic Cell-Based Vaccine For Cancer Therapy.- Evaluation of Bystander Cell Killing Effects in Suicide Gene Therapy of Cancer: Engineered Thymidylate Kinase (TMPK)/AZT Enzyme-Prodrug Axis.- Oncoleaking: Use of the Pore-forming Clostridium perfringens Enterotoxin (CPE) for Suicide Gene Therapy .- iCaspase 9 Suicide Gene System.- p53-encoding pDNA Purification by Affinity-Chromatography for Cancer Therapy.- A qRT-PCR Method for Determining the Biodistribution Profile of a miR-34a Mimic.- Design and Selection of Antisense Oligonucleotides Targeting Transforming Growth Factor Beta (TGF-b) Isoform mRNAs for the Treatment of Solid Tumors.- RNA Interference for Antimetastatic Therapy.- STAT3 Decoy ODN Therapy for Cancer.- Oncolytic Viral Therapy using Reovirus.- Use of GLV-1h68 for Vaccinia Virotherapy and Monitoring.- Back To The Future: Are Tumor-Targeting Bacteria The Next Generation Cancer Therapy?.- Ethics of Cancer Gene Transfer Clinical Research.- Planning an Academic Clinical Trial.- Production of Plasmid DNA as Pharmaceutical.- Minicircle - Next Generation DNA Vectors for Vaccination.- A Phase 2, Open-Label, Randomized Study of Pexa-Vec (JX-594) Administered by Intratumoral Injection in Patients with Unresectable Primary Hepatocellular Carcinoma.- AntiangiogenicMetargidin Peptide (AMEP) Gene Therapy in Disseminated Melanoma.- Clinical Evaluation of ErbB-targeted CAR T-cells, Following Intra-Cavity Delivery in Patients with ErbB-Expressing Solid Tumors.