Glaucoma

This second edition volume expands on the previous edition with an update on the latest methodologies used to study the neurodegeneration of glaucoma. This book notably features a focus on ocular hypertension as an important factor in the pathogenesis of glaucoma, and the relationship between elevated intraocular pressure and neurodegeneration. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Glaucoma: Methods and Protocols, Second Edition is a valuable resource for anyone interested in glaucoma research. This book will encourage innovation and facilitate progress toward improving our understanding and treatment of glaucoma.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

Evaluation of Cross-Linked Actin Networks (CLANs) in Human Trabecular Meshwork Cells and Tissues.- 3D Extracellular Matrix Protein Hydrogels for Human Trabecular Meshwork Cell Studies.- Protocol for the Extraction and Characterization of Trabecular Meshwork Cell Cytoskeletome Fraction.- Measurements of Phagocytosis in Trabecular Meshwork Cells.- Nanotubules and Cellular Communication in Trabecular Meshwork Cells.- Isolation and Characterization of Exosomes from Ocular Samples.- Timelapse Live-Cell Imaging Using Fluorescent Protein Sensors in Outflow Pathway Cells Under Fluid Flow Conditions.- Human Anterior Segment Perfusion Organ Culture.- Segmental Aqueous Humor Outflow.- Human Corneal Rim Perfusion Culture.- The Inducible TGF2 Ocular Hypertension Mouse Model.- Mouse Model of Glucocorticoid-Induced Glaucoma.- Trabecular Meshwork Stem Cells for Glaucoma Treatment.- Aqueous Humor Angiography.- Retinal Ganglion Cell Transplantation: Differentiation and Quantification Strategies.- Retinal Ganglion Cell Subtypes and Their Vulnerability in Glaucoma.- Full-Field Electroretinogram Responses in Rodent Models of Ganglion Cell Injury.- Testing Visual Function by Assessment of the Optomotor Reflex in Glaucoma.- Controlled Elevation of Intraocular Pressure (CEI) Glaucoma Model in Rats.- A Comprehensive Protocol for Microbead-Induced Ocular Hypertension in Mice.- Continuous Wireless Telemetric Measurement of Intraocular Pressure (IOP), Ocular Perfusion Pressure (OPP), and Cerebrospinal Fluid Pressure (CFP) in Nonhuman Primates (NHPs).- In Vitro and In Vivo Methods for Studying Retinal Ganglion Cell Survival and Optic Nerve Regeneration.