Globins and Other Nitric Oxide-Reactive Proteins, Part A: Volume 436

Informationen zum Autor Professor Robert K Poole is Emeritus Professor of Microbiology at the University of Sheffield, UK. He was previously West Riding Professor of Microbiology at Sheffield and until 1996 held a Personal Chair in Microbiology at King's College London. During his long career, he has been awarded several research Fellowships, and taken sabbatical leave at the Australian National University, Kyoto University and Cornell University. His career-long interests have been in the areas of bacterial respiratory metabolism, metal-microbe interactions and bioactive small gas molecules. In particular, he has made notable contributions to bacterial terminal oxidases and resistance to nitric oxide with implications for bacterial pathogenesis. He co-discovered the flavohaemoglobin Hmp, now recognised as the preeminent mechanism of nitric oxide resistance in bacteria. He has served as Chairman of numerous research council grant committees, held research grants for over 40 years and published extensively (h-index, 2024 = 70). He served on several Institute review panels in the UK and overseas. He is a Fellow of the Royal Society of Chemistry and the Royal Society of Biology. Klappentext The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 400 volumes (all of them still in print), the series contains much material still relevant today truly an essential publication for researchers in all fields of life sciences. Methods in Enzymology is now available online at ScienceDirect full-text online of volumes 1 onwards. This volume features methods for the study of globin and other nitric oxide-reactive proteins. Zusammenfassung Suitable for researchers in the field of life sciences! this book features methods for the study of globin and other nitric oxide-reactive proteins. Inhaltsverzeichnis Section I. Nitric Oxide: Chemical and Analytical Methods Chapter 1 The Chemistry of Nitric Oxide and Related Species Martin H. Hughes Chapter 2 Delivery of nitric oxide for analysis of the function of cytochrome c'. Lindsay J. Cole, Wilhelmina M. Huston, and James W.B. Moir Chapter 3 The preparation and purification of NO gas and the use of NO releasers. The application of NO donors and other agents of nitrosative stress in biological systems Rubina G. Aga and Martin N. Hughes Chapter 4 The Chemistry of Peroxynitrite - Implications for Biological Activity Sara Goldstein and Gabor Merényi Chapter 5 Nitric Oxide (NO) Selective Electrodes Ian R. Davies, Xueji Zhang Chapter 6 NO, N2O and O2 Reaction Kinetics: Scope and Limitations of the Clark Electrode L.A.M. Pouvreau, M. J. F. Strampraad, S. Van Berloo, J. H. Kattenberg and S. de Vries Chapter 7 Chemiluminescence quantification of Nitric oxide and its derivatives in liquid samples Jay R. Laver, Tânia M. Stevanin, Robert C. Read Section II. Bacterial and Archaeal Hemoglobins Chapter 8 Interactions of nitric oxide with hemoglobin: from microbes to man Michael Angelo, Alfred Hausladen and Jonathan S. Stamler Chapter 9 Expression and purification of E. coli Hmp Robert K. Poole Chapter 10 Structural studies on flavohemoglobins Andrea Ilari and Alberto Boffi Chapter 11 Flavohemoglobin of Staphylococcus aureus Lígia S. Nobre, Vera L. Gonçalves and Lígia M. Saraiva Chapter 12 Assay and Characterization of the Nitric Oxide Dioxygenase Activity of (Flavo)Hemoglobins Paul R. Gardner Chapter 13 Globin Interactions with Lipids and Membranes Antonio Di Giulio and Alessandra Bonamore Chapter 14 Assessment of Biot...

Autorentext

Professor Robert K Poole is Emeritus Professor of Microbiology at the University of Sheffield, UK. He was previously West Riding Professor of Microbiology at Sheffield and until 1996 held a Personal Chair in Microbiology at King's College London. During his long career, he has been awarded several research Fellowships, and taken sabbatical leave at the Australian National University, Kyoto University and Cornell University. His career-long interests have been in the areas of bacterial respiratory metabolism, metal-microbe interactions and bioactive small gas molecules. In particular, he has made notable contributions to bacterial terminal oxidases and resistance to nitric oxide with implications for bacterial pathogenesis. He co-discovered the flavohaemoglobin Hmp, now recognised as the preeminent mechanism of nitric oxide resistance in bacteria. He has served as Chairman of numerous research council grant committees, held research grants for over 40 years and published extensively (h-index, 2024 = 70). He served on several Institute review panels in the UK and overseas. He is a Fellow of the Royal Society of Chemistry and the Royal Society of Biology.

Klappentext

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 400 volumes (all of them still in print), the series contains much material still relevant today truly an essential publication for researchers in all fields of life sciences.
Methods in Enzymology is now available online at ScienceDirect full-text online of volumes 1 onwards.
This volume features methods for the study of globin and other nitric oxide-reactive proteins.

Zusammenfassung
Suitable for researchers in the field of life sciences, this book features methods for the study of globin and other nitric oxide-reactive proteins.

Inhalt

Section I. Nitric Oxide: Chemical and Analytical Methods

Chapter 1
The Chemistry of Nitric Oxide and Related Species
Martin H. Hughes

Chapter 2
Delivery of nitric oxide for analysis of the function of cytochrome c'.
Lindsay J. Cole, Wilhelmina M. Huston, and James W.B. Moir

Chapter 3
The preparation and purification of NO gas and the use of NO releasers. The application of NO donors and other agents of nitrosative stress in biological systems
Rubina G. Aga and Martin N. Hughes

Chapter 4
The Chemistry of Peroxynitrite - Implications for Biological Activity
Sara Goldstein and Gabor Merényi

Chapter 5
Nitric Oxide (NO) Selective Electrodes
Ian R. Davies, Xueji Zhang

Chapter 6
NO, N2O and O2 Reaction Kinetics: Scope and Limitations of the Clark Electrode
L.A.M. Pouvreau, M. J. F. Strampraad, S. Van Berloo, J. H. Kattenberg and S. de Vries

Chapter 7
Chemiluminescence quantification of Nitric oxide and its derivatives in liquid samples
Jay R. Laver, Tânia M. Stevanin, Robert C. Read

Section II. Bacterial and Archaeal Hemoglobins

Chapter 8
Interactions of nitric oxide with hemoglobin: from microbes to man
Michael Angelo, Alfred Hausladen and Jonathan S. Stamler

Chapter 9
Expression and purification of E. coli Hmp
Robert K. Poole

Chapter 10
Structural studies on flavohemoglobins
Andrea Ilari and Alberto Boffi

Chapter 11
Flavohemoglobin of Staphylococcus aureus
Lígia S. Nobre, Vera L. Gonçalves and Lígia M. Saraiva

Chapter 12
Assay and Characterization of the Nitric Oxide Dioxygenase Activity of (Flavo)Hemoglobins
Paul R. Gardner

Chapter 13
Globin Interactions with Lipids and Membranes
Antonio Di Giulio and Alessandra Bonamore

Chapter 14
Assessment of Biotechnologically Relevant Characteristics of Heterologous Hemoglobins in Escherichia coli
Pauli T. Kallio, Christian J. T. Bollinger, Taija Koskenkorva, and Alexander D. Frey

Chapter 15
Applications of the Vitreoscilla hemoglobin (VHb) gene (vgb) for improved microbial fermentation processes
Xiao-Xing WEI, Guo-Qiang CHEN

Ch…