Glycomics on pig derived human glycoproteins

Glycosylation of recombinant proteins is of
particular importance because it can play
significant roles in the clinical properties of the
glycoprotein, such as enzyme activity, protein
stability, pharmacokinetics, and immunogenicity. In
this work, the N-glycan structures of recombinant
human Factor IX (tg-FIX) and Protein C (tg-PC)
produced in the transgenic pig mammary gland were
determined. It has been found that the majority of N-
glycans of tg-FIX and tg-PC are complex bi- and tri-
antennary with one or two terminal N-
acetylneuraminic acid moieties. There were
significant species-specific and tissue/cell-
specific differences in N-glycan structures among
animals used for transgenic animal bioreactors. N-
glycan profiles of tg-FIX were consistent during
lactation with respect to the overall distribution
of sialylated vs. neutral oligosaccharides. The
results show that the porcine mammary gland can be a
viable candidate bioreactor for production of
recombinant human glycoproteins that require
complex, sialylated N-linked glycans.

Autorentext

Dr. Gil recieved Ph.D. in the field of Glycomics and Glycoproteomics at the University of Nebraska, Lincoln, NE, USA in 2006. Now, he continues to work on Glycomics research at Sandia National Laboratories, Livermore, CA, USA.

Klappentext

Glycosylation of recombinant proteins is of particular importance because it can play significant roles in the clinical properties of the glycoprotein, such as enzyme activity, protein stability, pharmacokinetics, and immunogenicity. In this work, the N-glycan structures of recombinant human Factor IX (tg-FIX) and Protein C (tg-PC) produced in the transgenic pig mammary gland were determined. It has been found that the majority of N-glycans of tg-FIX and tg-PC are complex bi- and tri-antennary with one or two terminal N-acetylneuraminic acid moieties. There were significant species-specific and tissue/cell-specific differences in N-glycan structures among animals used for transgenic animal bioreactors. N-glycan profiles of tg-FIX were consistent during lactation with respect to the overall distribution of sialylated vs. neutral oligosaccharides. The results show that the porcine mammary gland can be a viable candidate bioreactor for production of recombinant human glycoproteins that require complex, sialylated N-linked glycans.