Graft-Versus-Host Disease

This book seeks to provide an overview of traditional and emerging protocols used to examine the aetiology, mechanisms and pathophysiology of GVHD, as well as those used to identify novel biomarkers and to test existing and new therapies to prevent or treat GVHD. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, Graft-Versus-Host Disease: Methods and Protocols aims to serve as a useful resource for current and future researchers in these and other areas of health and disease.

Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts

Inhalt

Graft-Versus-Host Disease Mouse Models: A Clinical-Translational Perspective.- Xenogeneic Animal Models of Graft-Versus-Host Disease.- Utilisation of Clinical Data and Evaluation of Biomarkers in the Investigation of Graft-Versus-Host Disease Outcomes.- Endothelial Activation and Stress Index (EASIX) A Prognostic Marker for Mortality After Acute Graft-Versus-Host-Disease and Endothelial Complications.- Assessment of Survivorship in Allogeneic Haematopoietic Stem Cell Transplantation.- Allogeneic Mouse Models of Graft-Versus-Host Disease.- Transplantation and Monitoring of a Murine Minor Histocompatibility Antigen-Mismatched Model of Graft-versus-Host Disease.- A Clinically Relevant Mouse Model of Reduced Intensity Conditioning for Allogeneic HLA-identical Haematopoietic Stem Cell Transplantation.- Humanised Mouse Model of Graft-Versus-Host Disease Utilising Non-irradiated NOD-scid-IL-2Rnull Mice.- Canine Haematopoietic Cell Transplantation for Graft-Versus-Host Disease.- Flow Cytometry Analysis of Graft Conditioning on the T cell Response in Graft-versus-host Disease Following Murine Allogeneic Transplantation.- Mass Cytometry Immunophenotyping of Graft Conditioned Cells Following Major Histocompatibility Complex Mismatched Murine Allograft.- Mixed Leukocyte Reaction Using Human Blood Monocyte-Derived Dendritic Cells and Memory CD4+ T Cells.- Mixed Leukocyte Reaction Using Human Plasmacytoid Dendritic Cells and CD4+ T Cells.-Protocols for Studying Graft-Versus-Leukaemia Immunity in Mice.- Use of Human Chronic Myeloid Leukaemia Cells to Study Graft-versus-Leukaemia Immunity in a Xenogeneic Mouse Model of Graft-versus-Host Disease.