Guide to Yeast Genetics: Functional Genomics, Proteomics and Other Systems Analysis: Volume 470

Klappentext This fully updated edition of the best-selling three-part "Methods in Enzymology "series, "Guide to Yeast Genetics and Molecular Cell Biology "is specifically designed to meet the needs of graduate students, postdoctoral students, and researchers by providing all the up-to-date methods necessary to study genes in yeast. Procedures are included that enable newcomers to set up a yeast laboratory and to master basic manipulations. Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines. This volume serves as an essential reference for any beginning or experienced researcher in the field. Provides up-to-date methods necessary to study genes in yeast. Includes proceedures that enable newcomers to set up a yeast laboratory and to master basic manipulations. This volume serves as an essential reference for any beginning or experienced researcher in the field. Praise for the first edition: "...this is an excellent collection of methods that is certain to be an important handbook for yeast researchers."--Will Prinz, National Institutes of Health for ANALYTICAL BIOCHEMISTRY (April 2003) Zusammenfassung Part of the "Methods in Enzymology" series! this guide to yeast genetics and molecular cell biology intends to meet the needs of graduate students! postdoctoral students! and researchers by providing the various methods necessary to study genes in yeast. It is suitable for beginning or experienced researchers in the field. Inhaltsverzeichnis PART I - Functional Genomics 1.Analysis of Gene Function using DNA Microarrays Andrew P. Capaldi 2.An introduction to microarray data analysis and visualization Gregg B. Whitworth 3. Genome-wide approaches to monitor pre-mRNA splicing Maki Inada and Jeff Pleiss 4. ChIP-Seq: Using high-throughput DNA sequencing for genome-wide identification of transcription factor binding sites Philippe Lefrançois, Wei Zheng and Michael Snyder 5. Genome-wide mapping of nucleosomes in yeast Oliver J. Rando 6. Genome-wide translational profiling by ribosome footprinting Nicholas T. Ingolia PART II - Systematic Genetic Analysis 7. Synthetic Genetic Array (SGA) Analysis in Saccharomyces cerevisiae and Schizosaccharomyces pombe Anastasia Baryshnikova, Michael Costanzo, Scott Dixon, Franco J. Vizeacoumar, Chad L. Myers, Brenda Andrews and Charles Boone 8. Making temperature-sensitive mutants Shay Ben-Aroya, Xuewen Pan, Jef D. Boeke and Philip Hieter 9. Quantitative Genetic Interaction Mapping using the E-MAP Approach Sean R. Collins, Assen Roguev and Nevan J. Krogan 10. Exploring gene function and drug action using chemogenomic dosage assays Elke Ericson, Shawn Hoon, Robert P. St. Onge, Guri Giaever and Corey Nislow PART III - Proteomics 11. Yeast Expression Proteomics by High-Resolution Mass Spectrometry Tobias C. Walther, Jesper V. Olsen and Matthias Mann 12. High-quality binary interactome mapping Matija Dreze, Dario Monachello, Claire Lurin, Michael E. Cusick, David E. Hill, March Vidal, and Pascal Braun 13. Quantitative analysis of protein phosphorylation on a system-wide scale by mass spectrometry based proteomics Bernd Bodenmiller and Rudi Aebersold 14. A Toolkit of Protein-fragment Complementation Assays for Studying and Dissecting Large-scale and Dynamic Protein-Protein Interactions in Living Cells Stephen W. Michnick, Po-Hien Ear, Christian Landry, Mohan K. Malleshaiah and Vincent Messier 15. Yeast Lipid Analysis and Quantification by Mass Spectrometry Xue Li Guan, Isabelle Riezman, Markus R. Wenk and Howard Riezman 16. Mass Spectrometry-Based Metabolomics of Yeast Christopher A. Crutchfield, Wenyun Lu, Eugene Melamu...

Klappentext

This fully updated edition of the best-selling three-part Methods in Enzymology series, Guide to Yeast Genetics and Molecular Cell Biology is specifically designed to meet the needs of graduate students, postdoctoral students, and researchers by providing all the up-to-date methods necessary to study genes in yeast. Procedures are included that enable newcomers to set up a yeast laboratory and to master basic manipulations. This volume serves as an essential reference for any beginning or experienced researcher in the field.

Zusammenfassung
Part of the "Methods in Enzymology" series, this guide to yeast genetics and molecular cell biology intends to meet the needs of graduate students, postdoctoral students, and researchers by providing the various methods necessary to study genes in yeast. It is suitable for beginning or experienced researchers in the field.

Inhalt

PART I - Functional Genomics
1.Analysis of Gene Function using DNA Microarrays
Andrew P. Capaldi
2.An introduction to microarray data analysis and visualization
Gregg B. Whitworth

3. Genome-wide approaches to monitor pre-mRNA splicing
   Maki Inada and Jeff Pleiss
4. ChIP-Seq: Using high-throughput DNA sequencing for genome-wide identification of transcription factor binding sites
   Philippe Lefrançois, Wei Zheng and Michael Snyder
5. Genome-wide mapping of nucleosomes in yeast
   Oliver J. Rando

6. Genome-wide translational profiling by ribosome footprinting
   Nicholas T. Ingolia

   PART II - Systematic Genetic Analysis

7. Synthetic Genetic Array (SGA) Analysis in Saccharomyces cerevisiae and Schizosaccharomyces pombe
   Anastasia Baryshnikova, Michael Costanzo, Scott Dixon, Franco J. Vizeacoumar, Chad L. Myers, Brenda Andrews and Charles Boone
8. Making temperature-sensitive mutants
   Shay Ben-Aroya, Xuewen Pan, Jef D. Boeke and Philip Hieter
9. Quantitative Genetic Interaction Mapping using the E-MAP Approach
   Sean R. Collins, Assen Roguev and Nevan J. Krogan

10. Exploring gene function and drug action using chemogenomic dosage assays
    Elke Ericson, Shawn Hoon, Robert P. St. Onge, Guri Giaever and Corey Nislow

    PART III - Proteomics

11. Yeast Expression Proteomics by High-Resolution Mass Spectrometry
    Tobias C. Walther, Jesper V. Olsen and Matthias Mann
12. High-quality binary interactome mapping
    Matija Dreze, Dario Monachello, Claire Lurin, Michael E. Cusick, David E. Hill, March Vidal, and Pascal Braun
13. Quantitative analysis of protein phosphorylation on a system-wide scale by mass spectrometry based proteomics
    Bernd Bodenmiller and Rudi Aebersold
14. A Toolkit of Protein-fragment Complementation Assays for Studying and Dissecting Large-scale and Dynamic Protein-Protein Interactions in Living Cells
    Stephen W. Michnick, Po-Hien Ear, Christian Landry, Mohan K. Malleshaiah and Vincent Messier
15. Yeast Lipid Analysis and Quantification by Mass Spectrometry
    Xue Li Guan, Isabelle Riezman, Markus R. Wenk and Howard Riezman

16. Mass Spectrometry-Based Metabolomics of Yeast
    Christopher A. Crutchfield, Wenyun Lu, Eugene Melamud, and Joshua D. Rabinowitz

    PART IV - Systems Analysis

17. Imaging single mRNA molecules in yeast
    Hyun Youk, Arjun Raj and Alexander van Oudenaarden
18. Reconstructing gene histories in Ascomycota fungi
    IlanWapinski and Aviv Regev

19. Experimental evolution in yeast: a practical guide
    Maitreya J. Dunham

    PART V - Synthetic Biology

20. Enhancing stress resistance and production phenotypes through transcriptome engineering
    Felix H. Lam, Franz S. Hartner, Gerald R. Fink, and Gregory Stephanopoulos

    PART VI - Advances in Cytology/Biochemistry

21. Visualizing yeast chromosomes and nuclear architecture
    Peter Meister, Lutz R. Gehlen, Elisa Varela, Veronique Kalck and Susan M. Gasser
22. Quantitative localization of chromosomal loci by immunofluorescence
    Donna Garvey Brickner, William Light and Jason H. Brickner
23. Spinning Disk Confocal Microscopy of Yeast
    Kurt Thorn
24. Correlative GFP-immunoelectron microscopy in yeast
    Christopher Buser and Kent McDonald
25. Analyzing P-bodies and stress granules in Saccharomyces cerevisiae
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