Haplotyping

This volume details protocols for genetic, molecular, cytological, and bioinformatic methods for determining haplotypes. Haplotyping: Methods and Protocols guides readers through methods that directly type haploid cells, difficult-to-resolve gene families, high-resolution, short range haplotyping for targeted loci, and long-range haplotyping for whole chromosomes or genomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical Haplotyping: Methods and Protocols, aims to provide researchers with an overview of experimental methods for haplotyping.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

Haplotyping of Heterozygous SNPs in Genomic DNA using Long Range PCR.- Quantification and Sequencing of Crossover Recombinant Molecules from Arabidopsis Pollen DNA.- Pacbio for Haplotyping in Gene Families- High Molecular Weight DNA Enrichment with Peptide Nucleic Acid Probes.- High-Throughput Sequencing of the Major Histocompatibility Complex following Targeted Sequence Capture.- Pedigree-defined Haplotypes and their Applications to Genetic Studies.- Haplotyping a Non-Meiotic Diploid Fungal Pathogen using Induced Aneuploidies and SNP/CGH Microarray Analysis.- Whole-genome Haplotyping of Single Sperm of Daphnia Pulex (Crustacea, Anomopoda).- Chromosome-range Whole-genome High-throughput Experimental Haplotyping by single-chromosome Microdissection.- Phased Genome Sequencing through Chromosome Sorting.- Long Fragment Read (LFR) Technology: Cost-effective, High-quality Genome-wide Molecular Haplotyping.- Contiguity-Preserving Transposition Sequencing (CPT-seq) for Genome-Wide Haplotyping, Assembly, and Single-Cell ATAC-seq.- Fosmid Pool-Based Next Generation Sequencing to Haplotype-Resolve Whole Genomes.- Discovery of Rare-haplotype Variants by Typing Millions of Single-molecules with Bead Emulsion Haplotyping (BEH).- Computational Haplotype Inference from Pooled Samples.