Human Cytomegaloviruses

With the significant medical role that human cytomegalovirus plays in human disease (from acute disease following primary infection, to chronic disease due to life-long viral persistence), the need to better understand human cytomegalovirus biology and pathogenesis is needed. Human Cytomegaloviruses: Methods and Protocols is designed to be an inclusive document covering all of the techniques and approaches necessary to understand and study the pathobiology of human cytomegalovirus. Topics covered include the history of human cytomegaloviruses, techniques to culture and grow the virus in model cell types, the use of primary cells for the study of human cytomegalovirus pathogenesis, modern molecular techniques for assessing the biological consequences of viral infection, animal models for study of cytomegalovirus replication and numerous other topics of current interest. Written in the successful Methods in Molecular Biology series format, each chapter includes an introduction, list of necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Human Cytomegaloviruses: Methods and Protocols serves as a tool for basic scientists as well as clinical scientists with an interest in the basic fundamental aspects of viral gene expression and specific aspects of viral pathogenesis.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Inhalt

History of the Molecular Biology of Cytomegalovirus.- Overview of Human Cytomegalovirus Pathogenesis.- Distinct Properties of Human Cytomegalovirus Strains and the Appropriate Choice of Strains for Particular Studies.- Use of Diploid Human Fibroblasts as a Model System to Culture/Grow, Study and Understand Human Cytomegalovirus Infection.- Use of Recombinant Approaches to Construct Human Cytomegalovirus Mutants.- The Use of Primary Human Cells (Fibroblasts, Monocytes and Others) to Assess Human Cytomegalovirus Function.- Human Hematopoietic Long-Term Culture (hLTC) for Human Cytomegalovirus Latency and Reactivation.- Analysis of Cytomegalovirus Binding/Entry-Mediated Events.- Use of 5-Ethynyl-2'-Deoxyuridine Labelling and Flow Cytometry to Study Cell Cycle-Dependent Regulation of Human Cytomegalovirus Gene Expression.- Methods for Studying the Function of Cytomegalovirus GPCRs.- Methods for the Detection of Cytomegalovirus in Glioblastoma Cells and Tissues.- Methods to Study the Nucleocytoplasmic Transport of Macromolecules with Respect to Their Impact on the Regulation of Human Cytomegalovirus Gene Expression.- Fluorescence-Based Laser Capture Microscopy Technology Facilitates Identification of Critical In Vivo Cytomegalovirus Transcriptional Programs.- Techniques for Characterizing Cytomegalovirus-Encoded miRNAs.- What We Have Learned From Animal Models of HCMV.- Rodent Models of Congenital Cytomegalovirus Infection.- Recent Approaches and Strategies in the Generation of Anti-HumanCytomegalovirus Vaccines.- Approaches for the Generation of New Anti-Cytomegalovirus Agents: Identification of Protein-Protein Interaction Inhibitors and Compounds Against the HCMV IE2 Protein.