Imaging and Tracking Stem Cells

This fully updated book brings together protocols to arm stem cell biologists with tools and approaches to continue uncovering the intricacies and regulatory mechanisms underlying stem cell biology. Through various models and organ systems, the volume reflects the numerous recent advances in cell lineage and lineage tracking. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Imaging and Tracking Stem Cells: Methods and Protocols, Second Edition is an ideal guide for novices and experts alike who are working to expand our knowledge in the field of stem cells.

Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts

Inhalt

Molecular Imaging and Tracking Stem Cells in Neurosciences.- Tracking Germline Stem Cell Dynamics In Vivo in C. elegans Using Photoconversion.- Long-Term Cell Fate Tracking of Individual Renal Cells Using Serial Intravital Microscopy.- Tracking and Imaging of Transplanted Stem Cells in Animals.- Generation and Analysis of Pluripotent Stem Cell-Derived Cardiomyocytes and Endothelial Cells for High Content Screening Purposes.- Covisualization of Global DNA Methylation/Hydroxymethylation and Protein Biomarkers for Ultra-High Definition Epigenetic Phenotyping of Stem Cells.- A Distinctive MRI-Based Absolute Bias Correction Protocol for the Potential Labeling and In Vivo Tracking of Stem Cells in a TBI Mice Model.- Efficient Labeling of Human Mesenchymal Stem Cells Using Iron Oxide Nanoparticles.- Imaging and Tracking Stem Cell Engraftment in Ischemic Hearts by Near-Infrared Fluorescent Protein (iRFP) Labeling.- Long-Term Intravital Imaging of Cornea, Skin, and Hair Follicleby Multiphoton Microscope.- Cell Cycle Analysis Using In Vivo **Staining of DNA Synthesizing Cells.- Metabolic Labeling of Live-Stem Cell for In Vitro Imaging and In Vivo Tracking.- Study of Intracellular Cargo Trafficking and Co-Localization in the Phagosome and Autophagy-Lysosomal Pathways of Retinal Pigment Epithelium (RPE) Cells.- Timelapse Video-Microscopy and Single Cell Tracking to Study Neural Cell Behavior In Vitro.- Multiphoton Microscopy for Non-Invasive and Label-Free Imaging of Human Skin and Oral Mucosa Equivalents.- Molecular Imaging of Therapeutic Effect of Mesenchymal Stem Cell-Derived Exosomes for Hindlimb Ischemia Treatment.- In Vitro Methods to Simulate Pollution and Photo-Pollution Exposure in Human Skin-Epidermis.