Immunohistochemistry
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Immunohistochemistry or IHC refers to the process of localizing antigens (eg. proteins) in cells of a tissue section exploiting the principle of antibodies binding specifically to antigens in biological tissues. It takes its name from the roots "immuno," in reference to antibodies used in the procedure, and "histo," meaning tissue (compare to immunocytochemistry). Immunohistochemical staining is widely used in the diagnosis of abnormal cells such as those found in cancerous tumors. Specific molecular markers are characteristic of particular cellular events such as proliferation or cell death (apoptosis). IHC is also widely used in basic research to understand the distribution and localization of biomarkers and differentially expressed proteins in different parts of a biological tissue. Visualising an antibody-antigen interaction can be accomplished in a number of ways. In the most common instance, an antibody is conjugated to an enzyme, such as peroxidase, that can catalyse a colour-producing reaction (see immunoperoxidase staining). Alternatively, the antibody can also be tagged to a fluorophore, such as fluorescein, rhodamine, DyLight Fluor or Alexa Fluor.
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GarantieWeitere Informationen
- Allgemeine Informationen
- GTIN 09786130622763
- Editor Frederic P. Miller, Agnes F. Vandome, John McBrewster
- Genre Chemie
- EAN 9786130622763
- Format Fachbuch
- Titel Immunohistochemistry
- Herausgeber Alphascript Publishing
- Anzahl Seiten 76
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