Immunophenotyping

This volume presents the latest collection of immunophenotypic techniques and applications used in research and clinical settings. Chapters in this book cover topics such as constructions of high dimensions fluorescence and mass cytometry panels; fluorescence barcoding; using dried or lyophilized reagents; and immunophenotypic examples of specific cell types. The book concludes with a discussion on the critical roles of quality control and immunophenotyping in the clinical environment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Immunophenotyping: Methods and Protocols is a valuable resource for any researchers, clinician, or scientist interested in learning more about this evolvingfield.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
High Dimensional Immunophenotyping with Fluorescence-based Cytometry: A Practical Guidebook.- Immunophenotyping by Mass Cytometry.- Fluorescent Cell Barcoding for Immunophenotyping.- Immunophenotyping using Dried and Lyophilized Reagents.- Guidelines for Gating Flow Cytometry Data for Immunological Assays.- Strategies and Techniques for NK Cell Phenotyping.- Immunophenotyping of Human B Lymphocytes in the Blood and in the Adipose Tissue.- Multiparametric Flow Cytometry Analysis of Naïve, Memory, and Effector T Cells.- Immunophenotyping of Human Regulatory T Cells.- Immunophenotyping of Human Innate Lymphoid Cells.- Enumeration of Plasmacytoid Dendritic Cells in Peripheral Blood and Bone Marrow by Flow Cytometric Analysis.- Immunophenotyping of Circulating Endothelial Cell and Endothelial Microparticles.- Rare Event Phenotyping and Molecular Characterization: Circulating Tumor Cells.- Quality Control of Immunophenotyping.- Immunophenotyping of Acute Myeloid Leukemia.- Immunophenotyping of Acute Lymphoblastic Leukemia.- Clinical Flow Cytometry Testing in Chronic Lymphocytic Leukemia.- Immunophenotyping of Paroxysmal Nocturnal Hemoglobinuria (PNH).