Immunotoxicity Testing

This text explores recent critical advances in immunotoxicology. It provides current, lab-ready procedures along with the corresponding background information necessary to identify effective testing approaches for chemicals and drugs.

Recent critical advances in the field of immunotoxicology have led to incredible contributions to basic science, including crucial improvements in human risk assessment. In Immunotoxicity Testing: Methods and Protocols, expert researchers explore these changes, providing the reader with current, lab-ready procedures, along with the corresponding background information that is necessary to identify effective testing approaches for chemicals and drugs. Among the first volumes to meld consideration of immunotoxicity testing strategies with a comprehensive presentation of detailed laboratory protocols, chapters include a description of the evolution of immunotoxicity testing, a look at the importance of immunotoxicity testing for health risk reduction, and ideas concerning the future of the field. Composed in the highly successful Methods in Molecular Biology(tm) series format, most chapters contain a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips from the experts on troubleshooting and avoiding known pitfalls.

Comprehensive and innovative, Immunotoxicity Testing: Methods and Protocols is a critical, one-stop reference resource for the most important and commonly used laboratory protocols in immunotoxicology.

Features step-wise protocols for assays used in immunotoxicity safety evaluation Provides helpful hints to overcome assay problems or increased assay variability Includes chapters covering both clinical evaluation and alternative approaches Offers discussions of new assays likely to impact future testing protocols Presents discussions of age and gender considerations in immune safety testing Incorporates testing approaches designed to address emerging immune health risk concerns Includes supplementary material: sn.pub/extras

Zusammenfassung
Among the first volumes to meld consideration of immunotoxicity testing strategies with a comprehensive presentation of detailed laboratory protocols, chapters include a description of the evolution of immunotoxicity testing, a look at the importance of immunotoxicity testing for health risk reduction, and ideas concerning the future of the field.

Inhalt
Immunotoxicology Testing: Past and Future.- Overview and Health-Risk Considerations.- Developmental Immunotoxicity (DIT): The Why, When, and How of DIT Testing.- An In Vivo Tiered Approach to Test Immunosensitization by Low Molecular Weight Compounds.- Risk of Autoimmune Disease: Challenges for Immunotoxicity Testing.- Markers of Inflammation.- Evaluating Macrophages in Immunotoxicity Testing.- Immunotoxicity and Host Resistance Models.- Host Resistance Assays Including Bacterial Challenge Models.- Viral Host Resistance Studies.- Parasite Challenge as Host Resistance Models for Immunotoxicity Testing.- Tumor Challenges in Immunotoxicity Testing.- Testing Protocols in Rodents and Other Laboratory Animals.- The T-Dependent Antibody Response to Keyhole Limpet Hemocyanin in Rodents.- The Sheep Erythrocyte T-Dependent Antibody Response (TDAR).- The Delayed Type Hypersensitivity Assay Using Protein and Xenogeneic Cell Antigens.- The Cytotoxic T Lymphocyte Assay for Evaluating Cell-Mediated Immune Function.- NK Cell Assays in Immunotoxicity Testing.- The Local Lymph Node Assay and Skin Sensitization Testing.- Use of Contact Hypersensitivity in Immunotoxicity Testing.- Evaluation of Apoptosis in Immunotoxicity Testing.- Dendritic Cells in Immunotoxicity Testing.- Evaluating Cytokines in Immunotoxicity Testing.- Flow Cytometry in Preclinical Drug Development.- Enhanced Histopathology Evaluation of Lymphoid Organs.- Immunotoxicity Testing in Nonhuman Primates.- Evaluation in Humans.- Fundamentals of Clinical Immunotoxicology.- Wildlife Testing.- In Vivo Functional Tests for Assessing Immunotoxicity in Birds.- In Vitro Alternatives.- In Vitro Testing for Direct Immunotoxicity: State of the Art.